关键词: MDCK细胞, 牛血清, 贴壁培养, 生长曲线, 贴壁率

Abstract: In order to establish a rapid screening method for optimal serum of adherent culture cells, MDCK cells were serially subcultured in DMEM containing 10% different types of bovine serum, and observed by passage stability, growth curve and growth kinetics were analyzed. The method of learning and adherence rate was used to evaluate the effect of different types of bovine serum on the growth-promoting effect of MDCK adherent cells, and further expanded culture verification in cell factories. The results showed that all three types of serum could promote cell growth and proliferation and the cell growth curve was "S" type. MDCK cell growth kinetics analysis found that the average specific growth rate of the three types of serum was not significantly different (P>0.05); the maximum proliferation density and doubling time fetal bovine serum were superior to newborn bovine serum; the average colony formation rate analysis showed that, compared with the domestic fetal bovine serum(FBS), imported FBS made slightly difference (P=0.02), and the domestic newborn bovine serum was significantly lower than that of domestic and imported fetal bovine serum (P<0.0001). The domestic newborn bovine serum was used in subcultured MDCK cells in the 1st and 10th layer cell factories, the cells grew well, and the cell growth was stable during the passage. Studying the effects of different types of bovine serum on the culture of MDCK cells would provide a scientific basis for the expansion of MDCK cells in vaccine production and successful screening the best serum for MDCK cells in a short period of time.

Key words: MDCK cells, bovine serum, adherent culture, growth curve, adherent rate