关键词: 南方红豆杉, 干细胞培养, 培养基, 响应面分析

Abstract: Taxus cells culture is one of the effective ways to produce paclitaxel, and the growth rate and induction of paclitaxel production of cambial meristematic cells are better than that of the ordinary cells. The effects of different mass concentrations of GA3, citric acid, ascorbic acid and activated carbon on the growth and browning rate of Taxus chinensis cambial meristematic cells were studied by response surface methodology (RSM) on the basis of single factor experiment. Response surface methodology analysis showed that the optimal mass concentration of GA3 as 0.5 mg/L, citric acid as 150 mg/L, ascorbic acid as 100 mg/L and activated carbon as 100 mg/L in the cambial meristematic cells basal medium could promote the proliferation and inhibit the browning of Taxus chinensis cambial meristematic cells. This study laid the foundation for Taxus chinensis cambial meristematic cells culture to produce paclitaxel.

Key words: Taxus chinensis, cambial meristematic cell, culture medium, response surface analysis