生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 36-.doi: 10.3969/j.issn.2095-1736.2021.06.036

• 研究报告 • 上一篇    下一篇

Alicycliphilus denitrificans Ylb10的Cr(VI)还原性质研究

  

  1. 1. 湖北省生物酵素工程技术研究中心（三峡大学），宜昌 443002；
    2. 中国轻工业功能酵母重点实验室（三峡大学），宜昌 443002；
    3. 三峡大学 生物与制药学院，宜昌 443002
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 吕育财，副教授，硕士生导师，主要从事环境微生物学、微生物生物修复及有机废弃物资源再利用，E-mail：yucai.lyu@ctgu.edu.cn
  • 作者简介:左群，硕士研究生，研究方向环境功能微生物，E-mail：qun_1771919232@163.com
  • 基金资助:
    湖北省技术创新专项（重大项目）（No. 2019ABA114）

Study on the Cr(VI) reduction properties of Alicycliphilus denitrificans Ylb10

  1. 1. Hubei Engineering Research Center for Biological Jiaosu, China Three Gorges University, Yichang 443002, China;
    2.Key Laboratory of Functional Yeast, China National Light Industry, China Three Gorges University,Yichang 443002, China; 3. College of Biological and Pharmaceutical Sciences,China Three Gorges University, Yichang 443002, China
  • Online:2021-12-18 Published:2021-12-15

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摘要： 从求索溪底泥中，成功分离并获得一株具有高效还原Cr（VI）的菌株Ylb10。利用生理生化法和16S rDNA 测序法对Ylb10菌株进行鉴定。并采用二苯碳酰二肼分光光度法测定Cr（VI），对其还原特性进行探究。结果表明，Ylb10菌是革兰氏阴性菌，与Alicycliphilus denitrificans的16S rDNA相似率为98.07%。Ylb10能很好地利用α-羟基-丁酸、L-乳酸、丙酸、乙酸、L-天冬氨酸、L-谷氨酸、L-丙氨酸、L-丝氨酸等底物。Ylb10菌相比摇床培养条件，在静置培养时能更好地还原Cr（VI），最佳还原Cr（VI）的pH值为8~9。当Cr（VI）的浓度低于200 mg/L时，该菌株能够将Cr（VI）还原，其中50 mg/L Cr（VI） 18 h的还原率达到96.5%，100 mg/L Cr（VI） 24 h的还原率达到93.83%，200 mg/L Cr（VI）浓度60 h还原效率为99-06%。由此得出，Alicycliphilus denitrificans Ylb10菌是一株高效六价铬还原菌，在处理铬污染的水质及土壤方面有很高的应用潜力。

关键词: 脱氮嗜脂环物菌, Cr（VI）还原细菌, 重金属, 铬污染生物修复

Abstract: From the bottom mud of Qiusuo Creek, a strain Ylb10 with high efficiency in reducing Cr(VI) was successfully isolated and obtained. The Ylb10 strain was identified by physiological and biochemical methods and 16S rDNA sequencing method, and the Cr(VI) was determined by diphenylcarbazide spectrophotometry to investigate its reduction characteristics. The results showed that Ylb10 was Gram-negative, and the 16S rDNA similarity rate with Alicycliphilus denitrificans was 98.07%. Ylb10 could make good use of α-hydroxy-butyric acid, L-lactic acid, propionic acid, acetic acid, L-aspartic acid, L-glutamic acid, L-alanine, L-erine and other substrates.Compared that with the shaker culture conditions, Cr(VI) could be better reduced during standing culture, and the optimal pH of Cr(VI) reduction was 8-9. When the concentration of Cr(VI) was lower than 200 mg/L, the strain could reduce all Cr(VI), of which, at 50 mg/L Cr(VI) concentration, it had a reduction rate of 96.45% in 18 hours; at 100 mg/L Cr(VI ), it had the reduction rate of 93-83% in 24 hours, and the reduction efficiency at 200 mg/L Cr(VI) concentration in 60 hours was 99.06%. It could be concluded that Alicycliphilus denitrificans Ylb10 was a high-efficiency hexavalent chromium reducing bacteria, and It had high application potential in the treatment of chromium-contaminated water quality and soil.

Key words: Alicycliphilus denitrificans, Cr(VI) reducing bacteria, heavy metals, bioremediation of chromium pollution

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