生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 42-.doi: 10.3969/j.issn.2095-1736.2023.04.042

• 研究报告 • 上一篇    下一篇

荧光光谱法分析天然虾青素对H2 O2 的抗氧化能力

郑鑫鑫1,2， 黄　青1,2
  

  1. 1. 中国科学院合肥物质科学研究院， 合肥 230031；
    2. 中国科学技术大学 研究生院科学岛分院， 合肥 230026
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 通讯作者: 黄青，博士，研究员，研究方向为生物物理学，E-mail:huangq@ipp.ac.cn
  • 作者简介:郑鑫鑫，硕士，研究方向为生物工程，E-mail:zhxx97666@126.com
  • 基金资助:
    国家自然科学基金项目（No.11775272）

The antioxidant capacity of natural astaxanthin against H2 O2 analyzed by fluorescence spectrometry

ZHENG Xinxin1,2 , HUANG Qing1,2   

  1. 1. Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;
    2. Science Island Branch, Graduate School of USTC, Hefei 230026, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 为探究虾青素对H2O2的抗氧化能力，利用高香草酸（Homovanillic acid，HVA）在辣根过氧化物酶（Horseradish peroxidase，HRP）催化条件下被H2O2氧化产生强荧光信号的联苯二聚体的化学反应，探测和分析抗氧化剂对联苯二聚体荧光光谱的改变和影响，由此建立虾青素清除H2O2抗氧化能力测定的新方法，并将虾青素对H2O2的抗氧化作用与3种典型的抗氧化剂β-胡萝卜素、维生素C和α-生育酚对H2O2的抗氧化作用进行比较。结果表明，虾青素的H2O2清除能力明显高于β-胡萝卜素、维生素C和α-生育酚，约是β-胡萝卜素的1.4倍，是维生素C的8倍，是α-生育酚的732倍。基于荧光光谱法分析天然虾青素对H2O2的抗氧化能力，为虾青素抗氧化功能提供新的实验依据，也有利于人们对虾青素抗氧化功能的理解和应用发掘。

关键词: 抗氧化, 虾青素, 荧光光谱法, H2O2, 高香草酸（HVA）

Abstract: In order to explore the antioxidant capacity of astaxanthin to H2O2, a new method for determining the oxidation capacity of astaxanthin to remove H2O2was established by detecting and analyzing the changes and effects of antioxidants on the fluorescence spectrum of biphenyl dimers under the catalytic conditions of horseradish peroxidase (HRP) using homovanillic acid (HVA) to produce a strong fluorescent signal. And the antioxidant effect of astaxanthin against H2O2was compared with that of β-carotene, vitamin C and α-tocopherols. The results showed that the H2O2clearance capacity of astaxanthin was significantly higher than that of β-carotene, vitamin C and α-tocopherol, which was about 1.4 times that of β-carotene, 8 times that of vitamin C, and 732 times that of α-tocopherol. The antioxidation capacity of natural astaxanthin against H2O2was analyzed by fluorescence spectrometry, which provides new evidence for the excellent antioxidant function of astaxanthin, and is also conducive to people’s understanding and exploration of the antioxidant function of astaxanthin.

Key words: antioxidant, astaxanthin, fluorescence spectrometry, H2O2, homovanillic acid (HVA)

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