生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 8-.doi: 10.3969/j.issn.2095-1736.2022.05.008

• 研究报告 • 上一篇    下一篇

减数分裂型粘连蛋白RAD21L和REC8在DNA双链断裂修复中的影响

  

  1. 内蒙古师范大学生命科学与技术学院，呼和浩特010022
  • 出版日期:2022-10-18 发布日期:2022-10-18
  • 通讯作者: 美荣，博士，讲师，研究方向为发育生物学、细胞生物学，E-mail:meirong@imnu.edu.cn
  • 作者简介:乌云达来，博士，实验师，研究方向为发育生物学与表观遗传学，E-mail:wuyundalai@imnu.edu.cn
  • 基金资助:
    国家自然科学基金项目 (编号：31860327)；内蒙古自然科学基金项目 (编号：2020MS03093)；内蒙古自治区高等学校科学研究项目 (编号：NJZZ18019)；内蒙古师范大学引进高层次人才科研启动经费项目 (编号：2017YJRC021)

Effect of meiotic cohesins RAD21L and REC8 on DNA double strand break repair

  1. College of Life Science and Technology, Inner Mongolia Normal University, Huhhot 010022, China
  • Online:2022-10-18 Published:2022-10-18

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摘要： 为揭示减数分裂型粘连蛋白RAD21L和REC8在同源染色体重组过程中的作用机制，采用小鼠精母细胞，通过高分辨率显微镜（3D-SIM）观察RAD21L和REC8与重组中间体RAD51及MSH4之间的定位关系，并比较两种粘连蛋白与重组中间体的相关性。结果发现，RAD21L与REC8相比较，更倾向表达在重组中间体邻近之处，甚至横跨在还未联会的同源染色体的某些部位。这些部位往往是重组中间体的存在之处。这提示RAD21L间接作用于同源染色体的重组，当染色体上发生DNA双链断裂（double strand breaks，DSBs），RAD21L将聚集并固定在DSBs周围区域，以保证重组中间体顺利完成DSBs的修复，而对REC8，DSBs的修复则是次要功能。

关键词: 小鼠, 同源染色体的重组, DSBs修复, 粘连蛋白, RAD21L

Abstract: In order to reveal the molecular mechanisms in the process of homologous chromosome recombination, spermatocytes ofmouse were used to observe the localization relationship between RAD21L and REC8 and the recombination intermediates RAD51 and MSH4 by high-resolution microscope (3D-SIM), and to compare the correlation of the recombination intermediates between RAD21L and REC8. The results showed that RAD21L was more likely to express in the vicinity of recombinant intermediates than that of REC8, and even express insome parts of homologous chromosomes that had not yet been linked. Interestingly, these parts were just where recombinant intermediates existed. It is believed that RAD21L acted indirectly on the recombination of homologous chromosomes. When DSBs occurred on the chromosome, RAD21L would accumulate and fix around DSBs to ensure the successful repair of DSBs, while the repair of DSBs was a secondary function for REC8.

Key words: mouse, homologous chromosome recombination, DSBs repair, cohesin, RAD21L

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