生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (1): 39-.doi: 10.3969/j.issn.2095-1736.2020.01.039

• 研究报告 • 上一篇    下一篇

亲本引起断乳前小鼠的位置偏爱及神经内分泌反应

  

  1. 1. 北方民族大学 国家民委生态建模与应用重点实验室, 银川 750021 2. 北方民族大学 生物科学与工程学院, 银川 750021
  • 出版日期:2020-02-18 发布日期:2020-03-09
  • 通讯作者: 王建礼，博士,教授，研究方向为行为生理学，E-mail: wang_jianli@163.com
  • 作者简介:马小梅，硕士研究生，研究方向为行为生理学，E-mail: 621136721@qq.com
  • 基金资助:
    国家自然科学基金项目(31460565；31260513)；北方民族大学研究生创新项目

Place preference to a parent and neuroendocrine responses in preweanling mice

  1. 1. Key Laboratory of Ecology Model and Applications of State Ethnic Affairs Commission; 
    2. College of Biological Sciences and Engineering, North Minzu University, Yinchuan 750021, China
  • Online:2020-02-18 Published:2020-03-09

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摘要： 断乳前紧密的亲-子联系对于哺乳动物非常重要，这种联系促进了亲-子双方形成对彼此的情感依附，但关于幼体对亲本形成依附的机制还不清楚。精氨酸加压素（AVP）和皮质酮（CORT）在社会互作和社会联系中具有重要作用。为了探讨ICR品系小鼠幼体对母本或父本形成依附的可能机制，利用条件位置偏爱（CPP）实验检测了断乳前雄性幼体经过父鼠或母鼠4 d强化后的位置偏爱，利用免疫组织化学方法检测了形成位置偏爱时幼鼠下丘脑室旁核（PVN）和视上核（SON）AVP和c-Fos免疫活性神经元的表达，通过酶联免疫实验检测了血清CORT水平。结果发现，断乳前幼体能够产生对母鼠或父鼠信息箱的位置偏爱（P<0.05），与空箱强化相比，母鼠或父鼠强化均增加了幼体PVN的c-Fos阳性神经元表达（P<0.05），减少了血清CORT水平（P<0.05），而且经过母鼠强化的幼体PVN呈现出更多的AVP阳性神经元（P<0.05）。与父鼠强化的幼体相比，母鼠强化的幼体PVN的c-Fos阳性神经元较少（P<0.05），血清CORT水平较低（P<0.05）。SON的c-Fos和AVP免疫活性神经元没有显著性差异（P>0.05）。这些结果表明母鼠或父鼠强化可以引起幼鼠的位置偏爱，这种位置偏爱可能与PVN的神经元活动、AVP释放及血清CORT的改变有关，而且母鼠强化和父鼠强化引起幼鼠的神经内分泌活动不同。

关键词: ICR品系小鼠, 位置偏爱, 精氨酸加压素, 皮质酮, c-Fos

Abstract: The close parent-infant bonding before weaning is critical in mammals, which facilitates the emotional attachment between each other. However, the mechanisms on filial emotional attachment towards a parent remain poorly understood. Arginine vasopressin (AVP) and corticosterone (CORT) play important roles in social interaction and social bonding. This study aimed to investigate the potential mechanisms on parent-induced attachment in ICR mice pups through conditioned place preference (CPP) test. We investigated place preference of pre-weanling male pups following four days′ maternal or paternal reinforcing, while the immunoreactive (IR) neurons of hypothalamic AVP and c-Fos were detected by immunohistochemistry staining. In parallel, the levels of serum CORT were detected by enzyme-linked immunosorbent assay in mice pups. The pups conditioned with a parent showed place preference for the chamber paired with dam or sire cue with a corresponding increase in c-Fos expression in the paraventricular nucleus of the hypothalamus (PVN) and a decrease in serum CORT levels compare to the pups conditioned with empty chamber (P<0.05), and the pups conditioned with dams displayed higher levels of AVP expression in the PVN (P<0.05). In comparison with the pups conditioned with sires, the pups conditioned with dams showed lower levels of PVN c-Fos expression (P<0.05) and serum CORT concentration (P<0.05). There were not significant differences in c-Fos-and AVP-IR expression in the supraoptic nucleus of the hypothalamus (SON)(P>0.05). These results indicate the pre-weanling pups display place preference when conditioning with mothers or fathers. The neuronal activation and AVP release in the PVN and alterations of serum CORT may contribute to a parent induced-preference, and there were some differences with respect to the neuroendocrine activities of pups between maternal and paternal conditioning.

Key words: ICR mice, place preference, arginine vasopressin, corticosterone, c-Fos

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