生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 38-.doi: 10.3969/j.issn.2095-1736.2022.05.038

• 研究报告 • 上一篇    下一篇

一株产红色素沙雷氏菌基因表达谱差异分析

  

  1. 1. 北方民族大学 生物科学与工程学院, 银川 750021;
    2. 宁夏特殊生境微生物资源开发与利用重点实验室, 银川 750021
  • 出版日期:2022-10-18 发布日期:2022-10-18
  • 作者简介:李靖宇,博士,副教授,研究方向为环境微生物学,E-mail:lijingyu1986@126.com
  • 基金资助:
    国家自然科学基金项目(41661053); 中央高校基本科研业务费北方民族大学高层次人才引进科研启动项目(2019KYQ001); 宁夏特殊微生物资源开发与利用重点实验室研究项目(2018TSW04)

Differential analysis of gene expression profile of Serratia sp. producing red pigment

  1. 1. College of Biological Science & Engineering, North Minzu University, Yinchuan 750021, China; 2. Ningxia Key
    Laboratory of Microbial Resources Development and Applications in Special Environment, Yinchuan 750021, China
  • Online:2022-10-18 Published:2022-10-18

摘要: 利用转录组测序技术对分离纯化获得的一株产红色素沙雷氏菌在不同生长阶段(产色素和不产色素)中基因表达谱差异特征进行分析,该菌株不同生长阶段基因表达谱共获得显著差异基因2512个,其中:显著上调基因1317个,基于该基因集的KEGG Pathway富集分析表明,参与群体感应功能的基因存在显著富集趋势,产色素阶段高表达自诱导物AI-2激酶lsrK以及LuxR家族转录调节因子sdiA;显著下调基因1195个,基于该基因集的KEGG Pathway富集分析表明,参与核糖体、鞭毛组装、TCA循环、肽聚糖生物合成、氨酰tRNA生物合成、原核生物碳固定、阳离子抗菌肽耐药性、细菌趋化性、细菌分泌系统、氧化磷酸化,以及丙氨酸、天冬氨酸和谷氨酸代谢等功能的基因存在显著富集趋势。

关键词: 红色素;沙雷氏菌;转录组;基因表达谱差异, 群体感应

Abstract: Transcriptome sequencing was used to deeply understand the gene expression profile of a strain of Serratia producing red pigment at different growth stages (pigment and non-pigment producing) which was isolated and purified in the laboratory. A total of 2512 differentially expressed genes were obtained from the gene expression profiles of different growth stages, of which 1317 were significantly up-regulated. Pathway enrichment analysis showed that there was a significant enrichment trend in quorum sensing function. In the pigment production stage, the AI-2 kinase lsrk was highly expressed, and the LuxR family transcription regulator sdiA was also highly expressed. And 1195 genes were significantly down regulated, and pathway enrichment analysis showed that there were significant enrichment trends in ribosome, flagellum assembly, TCA cycle, peptidoglycan biosynthesis, aminoacyl tRNA biosynthesis, prokaryotic carbon fixation, cationic antimicrobial peptide resistance, bacterial chemotaxis, oxidative phosphorylation, alanine, aspartate and glutamate metabolism, and bacterial secretion system.

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