• 研究报告 •

### 抗MRSA植物药组方MZL-1的药效作用及分子机制

1. 1. 西南交通大学 生命科学与工程学院， 成都 610031；
2. 四川省医学科学院/四川省人民医院， 成都 610072
• 出版日期:2022-10-18 发布日期:2022-10-18
• 通讯作者: 茆灿泉，博士，教授，主要从事生物医药研发，E-mail: maocq@swjtu.edu.cn
• 作者简介:李栋，硕士，研究方向为创新中药及药理方向，E-mail: 851839723@qq.com
• 基金资助:
中央高校基本科研业务费（A0920502052001-9）

### Inhibitory effect and molecular mechanism of medicinal plants formula MZL-1 against MRSA

1. 1. School of Life Science and Engineering，Southwest Jiaotong University，Chengdu 610031, China;
2. Sichuan Academy of Medical Sciences/ Sichuan Provincial People’s Hospital, Chengdu 610072, China
• Online:2022-10-18 Published:2022-10-18

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) was used to study the anti-MRSA effect and preliminary molecular mechanism of the medicinal plant formula MZL-1 developed in the laboratory. The antibacterial effect of MZL-1 was confirmed by the double dilution method, and the relative conductivity, alkaline phosphatase activity change and scanning electron microscope observation were detected to analyze the effect of MZL-1 treatment on the destruction and integrity of the cell wall and membrane of MRSA. Flow cytometry was utilized to determine the effect of MZL-1 on MRSA for cell apoptosis. Network pharmacology was applied to explore the possible molecular mechanism of the anti-microbial effects of MZL-1. The result showed that the MIC of MZL-1 to MRSA was 7.5%. The increase in the activities of relative electric conductivity and alkaline phosphatase as well as the significant changes in morphology and damages observed by scanning electron microscopy indicated that MZL-1 obviously disrupted the integrity of MRSA cells and hence caused the overflow of the cell contents. Flow cytometry analysis found that a large number of cell apoptosis occurred in the early stage. Network pharmacology discovered 29 compounds and 23 target genes for MZL-1 in anti-microbial infection, and RELA may be the core gene. Gene Ontology(GO) enrichment analysis showed that target genes were mainly involved in the cell's response to small molecules, cytokines and transcription factors. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis found that the pathways involved mainly focused on inflammation, apoptosis, immune function, and bacterial and viral infections. Based on the current research, the formula MZL-1 might be potentially developed to be a plant-derived drug against MRSA and other drug-resistant pathogens and microbial infections.