生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (3): 88-.doi: 10.3969/j.issn. 2095-1736.2022.03.088

• 研究报告 • 上一篇    下一篇

响应面法制备黑曲霉浸膏水分散片剂及抗病促生作用

  

  1. 1.贵州大学酿酒与食品工程学院，贵阳550025； 2. 贵州大学生命科学学院，贵阳550025；
    3.贵州省产品质量检验检测院，贵阳550003
  • 出版日期:2022-06-18 发布日期:2022-06-17
  • 通讯作者: 李祝，教授，主要从事微生物与生化药物研究，E-mail：zhuliluck@163.com
  • 作者简介:姚蒋庞，硕士研究生，研究方向为应用微生物，E-mail：yaojiangpang@yeah.net

Preparation of Aspergillus niger extract water dispersible tablets by response surface methodology and study on its anti-disease and growth-promoting effects

  1. 1.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; 2. College of Life Science,
    Guizhou University, Guiyang 550025, China; 3. Institute of Products Detection ＆ Determination of Guizhou Province,
    Guiyang 550003, China
  • Online:2022-06-18 Published:2022-06-17

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摘要： 以黑曲霉浸膏为试验材料，采用单因素试验筛选和响应面分析法优化制备黑曲霉浸膏水分散片剂，通过田间应用研究黑曲霉浸膏水分散片剂对烟草农艺性状和生理生化指标的影响。结果表明：黑曲霉浸膏水分散片剂最佳配方为10%黑曲霉浸膏，崩解剂比例(酸碱)0.45，36%崩解剂，6%聚乙二醇6000，2%聚乙烯吡咯烷酮K30，填料补足；在此条件下制备得到的黑曲霉浸膏水分散片剂表面光滑，颗粒强度适中，黏冲不明显，悬浮率80.56%，pH5.71，崩解时间130.2s，符合农药剂型质量技术指标。通过田间试验结果表明：与对照相比，两地区施用黑曲霉浸膏水分散片剂烟草叶片数增加68.35%~89.83%、叶长增长9.54%~30.18%、叶宽增宽23.37%~29.38%、茎粗增粗66.40%~97.36%、株高增高5.80%~16.30%；在黑曲霉浸膏水分散片剂的作用下，对烟草青枯病的大田防治效果达50%以上，提高烟草对外界不利环境的适应能力，促进植株生长及环境抗逆性，为微生物菌剂的开发与应用奠定基础。

关键词: 黑曲霉浸膏, 水分散片剂, 农艺性状, 生理生化指标, 抗病

Abstract: Using Aspergillus niger extract as the test material, single factor test screening and response surface analysis were used to optimize the preparation of Aspergillus niger extract water-dispersible tablets, and the effects of Aspergillus niger extract water-dispersible tablets on tobacco agronomic characteristics and physiological and biochemical indicators were studied through field applications impact. The results of the study showed that the best formula for water-dispersible tablets of Aspergillus niger extract was: 10% Aspergillus niger extract, disintegrant ratio (acid-base) 0.45, 36% disintegrant, 6% polyethylene glycol 6000, 2% Polyvinylpyrrolidone K30, filled with fillers, the Aspergillus niger extract water-dispersible tablets prepared under these conditions had a smooth surface, moderate particle strength and low viscosity with suspension rate 80.56%, pH 5.71, and disintegration time 130.2 s. It met the quality and technical indicators of pesticide formulations. The results of field experiments showed that compared with the control, the number of leaves of tobacco using Aspergillus niger extract water-dispersible tablets increased by 68.35%-89.83%, the leaf length increased by 9.54%-30.18%, the leaf width increased by 23.37%-29.38%, and the stem diameter was increased by 23.37%-29.38%. The thickness increased by 66.40%-97.36% and the plant height increased by 5.80%-16.30%; under the action of the tablet, the field control effect of tobacco bacterial wilt reached more than 50%, it improved the adaptability of tobacco to the unfavorable environment and promoted plant growth and environmental resistance.

Key words: Aspergillus niger extract, water-dispersible tablet, agronomic traits, physiological and biochemical indexes, anti-disease

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