关键词: 蛋白酶, 土霉素菌渣, 接种量, 酶活, 转化率

Abstract: This paper selected two strains of terramycin resistant mold M-1 and M-2 obtained from the compost of terramycin producing bacteria residues. The acid, alkali and terramycin tolerance of the two strains was studied. The terramycin producing slag was used as the main medium component, the protease production capacity of the two strains along with conversion capacity of the slag producing bacteria of the active ingredient was investigated. The results showed that M-1 and M-2 both had good growth at pH 3 to 7. The acid resistance of mold M-1 was better than that of strain M-2. There was no obvious inhibitory effect on the growth of the two strains, when the concentration of terramycin was below 1800 u/mL. When the strains were cultured with 5% bacterial residue suspension medium, the two strains displayed good protease production characteristics. Under the optimal culture conditions, the protease production activity of M-1 reached to 99.33 u/mL, and M-2 reached to 44.41 u/mL. The optimal bacteria residue transformation conditions for strains M-1 and M-2 were 40 mL/250 mL liquid volume, 10% bacterial residue addition, 5% inoculum volume, pH 6.0, 30 ℃, shaker rotation cultivate at 160r/min for 168 h; and 100 mL/250 mL for filling, add 5% of bacterial residue, 9% of inoculum, pH 6.5, 30 ℃ shaker at 160 r/min for 144 hours, respectively.

Key words: protease, terramycin bacteria residue, inoculum, enzyme activity, conversion