生物学杂志 ›› 2022, Vol. 39 ›› Issue (3): 30-.doi: 10.3969/j.issn. 2095-1736.2022.03.030

• 研究报告 • 上一篇    下一篇

新型二维纳米材料Ti3C2/Co的抗菌及生物安全性研究

  

  1. 北京大学 口腔医院, 北京 100081
  • 出版日期:2022-06-18 发布日期:2022-06-17
  • 通讯作者: 贾绮林,博士,教授,主要从事口腔正畸学及材料抗菌的研究,E-mail: yljia2000@yahoo.com;郑云飞,博士,副研究员,主要从事口腔正畸学及材料抗菌的研究,E-mail: cora2005@126.com
  • 作者简介:周璇,硕士,主要从事二维层状纳米材料抗菌的研究,E-mail: ortho-zhouxuan@pku.edu.cn
  • 基金资助:
    国家自然科学基金青年项目(81700938)

Study on the antibacterial properties and biological safety of Ti3 C2/Co two-dimensional nanomaterials

  1. School of Stomatology,Peking University, Beijing 100081, China
  • Online:2022-06-18 Published:2022-06-17

摘要:  以大肠杆菌(E.coli)作为研究对象,通过标准平板计数法、活死菌染色法以及扫描电子显微镜镜下观察细菌表面结构来研究Ti3C2/Co纳米材料在近红外光照射下的抗菌性能,同时进一步通过溶血实验、脐静脉内皮细胞的cck8细胞毒性试验及迁移试验来验证其生物安全性。结果表明:Ti3C2/Co纳米材料在扫描电子显微镜下呈明显的片层状结构;钴元素的加入使材料表现出优异的磁性性能;Ti3C2/Co复合纳米材料具有良好的抗菌性能,在250 μg/mL浓度结合近红外光照射时其杀菌率可达到100%;而且Ti3C2/Co复合纳米材料在一定浓度范围内表现出优异的细胞相容性。材料具有潜在的广阔应用前景,可以制成抗菌涂层应用于医疗导管、手术器械及防菌纱布中。

关键词:  , Ti3C2 , /Co复合纳米材料; 近红外光; 抗菌活性; 生物相容性; 抗菌剂

Abstract: This experiment took E. coli as the research target flora. The antibacterial properties of Ti3C2/Co nanomaterials under near-infrared light (NIR) were studied by standard plate counting, live-dead bacteria staining, and observation of the bacterial surface structure under a scanning electron microscope. Hemolysis test, cck8 cytotoxicity test and migration test of umbilical vein endothelial cells were further verified its biological safety.The results showed that Ti3C2/Co nanomaterials were clear lamellar structures. The magnetic cobalt nanowires endowed Ti3C2/Co nanomaterials with controllable magnetic ability. Ti3C2/Co composite nanomaterials had good antibacterial properties, and its sterilization rate could reach 100% at a concentration of 250 μg/mL when combined with near-infrared light. At the same time, Ti3C2/Co composite nanomaterials showed excellent cell compatibility within a certain concentration range. This material had potential application prospects and could be used as an antibacterial agent in many fields such asmedical catheters, surgical instruments and antibacterial gauze.

Key words: Ti3C2/Co composite nanomaterials, near-infrared light(NIR), antibacterial activity, biological safety, antibacterial agent

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