生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 43-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 043

• 研究报告 • 上一篇    下一篇

全球海洋病毒数据库中新型噬病毒体的发现

  

  1. 1.上海海洋大学食品学院,上海201306; 2.农业农村部水产品质量安全贮藏保鲜风险评估实验室(上海),上海201306
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 通讯作者: 王永杰,博士,教授,研究方向为环境与食品病毒学,E-mail:yjwang@shou.edu.cn
  • 作者简介:周　亮,硕士研究生,研究方向为环境病毒学,E-mail:2606588402@qq.com
  • 基金资助:
    基金项目:国家自然科学基金项目(41376135,31570112)

Novel virophages discovered in global ocean viromes

  1. 1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;2. Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai),Ministry of Agriculture and Rural Affairs, Shanghai 201306, China
  • Online:2021-08-18 Published:2021-08-18

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摘要：

噬病毒体是寄生于原生生物巨病毒的一类小型双链DNA 病毒。带有噬病毒体的巨病毒侵染单细胞真核宿摘主的要能力下降,结果使得真核宿主的存活率上升。噬病毒体最早分离自巨病毒和单细胞真核宿主的共培养体系。随后基于宏基因组学分析表明,噬病毒体广泛分布于全球水环境中(尤其是淡水环境中)。通过深度分析全球海洋病毒数据库(Global Ocean Viromes) 序列,挖掘出了3 条完整的、新的噬病毒体全基因组序列( Global Ocean Viromes Virophage_1 /2/3, GOV_V1 /2/3)。系统发育和基因组分析表明,GOV_V1 与藻类巨病毒的潜在噬病毒体( 如QLV 和YSLV1,4,6) 亲缘关系最近,GOV2_V2 和GOV2_V3 则与阿米巴虫巨病毒的噬病体(Sputnik 家族) 亲缘性最近。结果显示,微藻可能是GOV2_V1 的真核宿主,而原生动物可能是GOV2_V2 和GOV2_V3 的真核宿主。


关键词: 噬病毒体, 全球海洋病毒数据库, 宏病毒组

Abstract: Virophages are small double-stranded DNA viruses that parasitize giant viruses of protists. With the co-existence of viroph-ages, giant viruses decrease the infection of single-cell eukaryotic hosts, resulting in increased survival rates of eukaryotic hosts. Vir-ophage were firstly isolated from the co-culture system of giant viruses and eukaryotic hosts. Subsequently, metagenomic analysisshowed that they widely distributed in the global aquatic environments, especially in fresh water habitats. Here complete genomic se-quences of three novel virophages (Global Ocean Viromes Virophage_1 /2/3, GOV_V1 /2/3) were identified through in-depth analysis of the Global Ocean Viromes datasets. Phylogenetic and genomic analyses indicated that GOV_V1 is most closely related to potential al-gae virus-infecting virophages (such as QLV and YSLV1,4,6), and GOV2_V2 /3 are mostly related to amoeba-infecting virophages (Sputnikvirus). These findings indicated that microalgae may be the hosts of GOV2_V1, while protozoan may be the hosts of GOV2_ V2 / 3.

Key words: virophage, global ocean viromes, metavirome

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