生物学杂志

生物学杂志

• 技术方法 • 上一篇    下一篇

多拉菌素产生菌的诱变及其发酵研究

  

  1. 江苏凌云药业， 常州 213200
  • 出版日期:2016-10-18 发布日期:2016-10-18
  • 作者简介:朱皖宜，高级工程师, 研究方向为工业微生物育种， E-mail:zwyhz@hotmail.com

Mutation breeding of doramectin mutant strain and study on fermentation

  1. Jiangsu Lingyun Pharmaceutical Company, Changzhou 213200, China
  • Online:2016-10-18 Published:2016-10-18

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摘要： 用实验室保藏的阿维链霉菌Streptomyces arermitilis 13＃为出发菌种，经过诱变选育获得高产突变株，并研究该菌株及工业化发酵工艺。经连续诱变，结合配方改良和发酵罐上工艺优化，获得的高产突变株其生产能力比出发株提高了127%，采用连续或间歇流加补糖的方式可延长产素周期，适合工业化生产。此突变株及发酵工艺的确定为产业化生产提供了参考。

关键词: 多拉菌素, 诱变, 发酵

Abstract: The mutant strain 1692＃was obtained from an original strain Streptomyces arermitilis 13＃using strain mutation screening method. Within the optimizing of the fermentation medium composition and the fermentation process, the production of the mutant strain was 127% higher than that of the original one. The mutant strain and the optimized fermentation process would provided a reference for the industrialized production of doramectin.

Key words: doramectin, mutation, fermentation