生物学杂志

生物学杂志

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三种培养基对小球藻生长的影响及在淡水轮虫培育中的应用研究

运用小球藻培养基SE、BG11和复合肥（CF）进行小球藻的培养，研究不同培养基对小球藻生长的影响，并研究5种不同浓度的CF培养基对小球藻及淡水轮虫培育的影响。结果表明，三种培养基培养小球藻均可取得较好的效果，培养1天和7天时，BG11效果最好，CF次之，SE最差，培养16、20和24天，BG11效果最好，SE次之，CF最差，差异均极显著。5不同浓度CF培养基中以60mg/L的培养基，培养小球藻和淡水轮虫效果最好，培养的淡水轮虫密度可达324.67±37.22ind/ml, 且与其他浓度的培养差异显著。   

  1. 浙江省淡水水产研究所，农业部淡水渔业健康养殖重点实验室，浙江省淡水水产遗传育种重点实验室，浙江 湖州，313001
  • 出版日期:2016-02-18 发布日期:2016-02-18
  • 通讯作者: 李飞，助理研究员，硕士，主要从事水产动物遗传育种与繁殖研究

 
Effects of Three Culture Media on the Growth of Chlorella and Its Application in Culture of Freshwater Rotifers

 

 SE, BG11 and CF(compound fertilizer) were used to culture Chlorella, to study their effects on growth of Chlorella, and the effects of five different concentration of CF were studied on the culture of freshwater rotifers. The results indicated that all the three culture media could obtain good culture in Chlorella. In 1 and 7 days culture, BG11 was the best, CF was followed, SE was the last; in 16, 20 and 24 days culture, BG11 was the best, SE was followed, CF was the last; the differences were significant. 60mg/L was the best in the five concentration of CF in culture of Chlorella and freshwater rotifers, the concentration of rotifers was 324.67±37.22ind/ml,and it was significantly different from other groups.
  

  1.  
    Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture，
    Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang province,
    Zhejiang Institute of Freshwater Fisheries，Huzhou 313001, China
  • Online:2016-02-18 Published:2016-02-18

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摘要：  

运用小球藻培养基SE、BG11和复合肥（CF）进行小球藻的培养，研究不同培养基对小球藻生长的影响，并研究5种不同浓度的CF培养基对小球藻及淡水轮虫培育的影响。结果表明，三种培养基培养小球藻均可取得较好的效果，培养1天和7天时，BG11效果最好，CF次之，SE最差，培养16、20和24天，BG11效果最好，SE次之，CF最差，差异均极显著。5不同浓度CF培养基中以60mg/L的培养基，培养小球藻和淡水轮虫效果最好，培养的淡水轮虫密度可达324.67±37.22ind/ml, 且与其他浓度的培养差异显著。

关键词: 培养基, 小球藻, 淡水轮虫

Abstract:  

SE, BG11 and CF(compound fertilizer) were used to culture Chlorella, to study their effects on growth of Chlorella, and the effects of five different concentration of CF were studied on the culture of freshwater rotifers. The results indicated that all the three culture media could obtain good culture in Chlorella. In 1 and 7 days culture, BG11 was the best, CF was followed, SE was the last; in 16, 20 and 24 days culture, BG11 was the best, SE was followed, CF was the last; the differences were significant. 60mg/L was the best in the five concentration of CF in culture of Chlorella and freshwater rotifers, the concentration of rotifers was 324.67±37.22ind/ml,and it was significantly different from other groups.