生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (5): 88-.doi: 10.3969/j.issn.2095-1736.2026.05.088

• 技术方法 • 上一篇    下一篇

N ,N-二甲基乙酰胺高效降解菌筛选及应用

杨园园， 雷　婷， 沈　超
  

  1. 贵州省材料产业技术研究院， 贵阳 550025
  • 出版日期:2026-10-18 发布日期:2026-10-20
  • 作者简介:杨园园，博士，副研究员，研究方向为水污染控制与治理，E-mail：544735956@qq.com
  • 基金资助:
    贵州省科技支撑计划项目（20204Y021）； 贵州省千层次人才计划项目（2025013）

Screening and application of high-efficiency degradation bacteria for N,N-dimethylacetamide

YANG Yuanyuan, LEI Ting, SHEN Chao   

  1. Guizhou Material Industry Technology Research Institute, Guiyang 550025, China
  • Online:2026-10-18 Published:2026-10-20

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摘要： 为解决制膜废水中N ，N-二甲基乙酰胺难以生化降解的问题，从贵阳市麦架桥污水处理厂的曝气池中取得活性污泥，通过驯化、筛选分离出1株N ，N-二甲基乙酰胺（DMAc）降解菌，命名为DC-1。根据菌落形态观察、生理特征分析，结合16S rDNA基因同源性对比，经鉴定，该菌为节杆菌属（Arthrobacter），推测为原玻璃蝇节杆菌（Arthrobacter protophormiae）。在考察单因素实验的基础上，使用Box-Behnken设计的响应面法优化菌株培养条件，如接种量、培养温度、pH，考察两两交互作用对DC-1降解DMAc性能的影响。结果表明，该菌生长和降解DMAc的适宜温度为29 ℃，pH为7.8，最佳接种量为13%。在最适宜的培养条件下，菌株DC-1在144 h内对初始体积分数为2% DMAc的去除率达到 90%以上。此外，DC-1菌株对同系物N ，N-二甲基甲酰胺（DMF）也表现出一定的降解能力，研究结果对高效降解制膜废水具有重要的应用价值。

关键词: 原玻璃蝇节杆菌； 降解菌； 制膜废水； N , N-二甲基乙酰胺； 降解特性

Abstract: To address the issue of biochemical degradation of N, N-dimethylacetamide （DMAc） in membrane wastewater, a bacterial strain designated DC-1, which is capable of effectively degrade DMAc, was isolated from the activated sludge of the Guiyang Maijiaqiao municipal wastewater treatment plant. Based on morphological observations, physiological and biochemical characterization, as well as phylogemetic analysis of the 16S rDNA gene sequence, it was identified asArthrobacter protophormiae. The effects of inoculum size, incubation temperature, and pH on the efficiency of DMAc degradation by DC-1 were investigated using a one-factor-at-a-time approach followed by response surface methodology based on the Box-Behnken design. The results indicated that the optimal temperature for bacterial growth and DMAc degradation was 29 ℃, the optimal pH was 7.8, and the optimal inoculum size was 13%. Under the optimized conditions, a degradation rate of over 90% was achieved for DMAc at an initial volume fraction of 2% within 144 h. Additionally, the strain exhibited certain degradation capabilities for the structurally similar compound dimethylformamide（DMF）. These findings demonstrated that strain DC-1 held considerable potential for the bioremediation of DMAc-contaminated wastewater, particularly in membrane manufacturing industries.

Key words: Arthrobacter protophormiae, degrading bacteria, membrane-making wastewater, N, N-dimethylacetamide, degradation characteristics

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