生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (5): 87-.doi: 10.3969/j.issn.2095-1736.2025.05.087

• 研究报告 • 上一篇    下一篇

场沼液中四环素对菌藻共生污水处理系统的影响

程俊芳1， 陈恒源1， 郑华宝1,2， 王　敏1,2   

  1. 1. 浙江农林大学 环境与资源学院， 杭州 311300；
    2. 浙江农林大学 环境与资源学院 浙江省土壤污染生物修复重点实验室， 杭州 311300
  • 出版日期:2025-10-18 发布日期:2025-10-14
  • 通讯作者: 王敏，博士，讲师，主要从事污水资源化处理及环境微生物研究，E-mail：wmin@zafu.edu.cn
  • 作者简介:程俊芳，硕士研究生，主要从事菌藻污水处理技术研究，E-mail：1732231988@qq.com
  • 基金资助:
    国家自然科学基金项目（32200084）

The effects of tetracycline in piggery digestate on microalgal bacterial consortia wastewater treatment #br#

CHENG Junfang1, CHEN Hengyuan1, ZHENG Huabao1,2, WANG Min1,2   

  1. 1. School of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300,
    China; 2. Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, School of Environmental
    and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
  • Online:2025-10-18 Published:2025-10-14

摘要： 研究四环素质量浓度以及四环素降解菌Serratia entomophilastrain TC-1对菌藻共生系统污染物去除率的影响，并通过扫描电镜观察和高通量测序技术，进一步分析微生物群落结构的变化，从而揭示在四环素胁迫下菌藻共生系统的污染物去除规律和微生物响应机制。研究结果表明，四环素质量浓度越高，微藻的生物量越低，菌藻共生系统的氨氮去除率越低，当四环素质量浓度为50 mg/L时，叶绿素质量浓度为19.19 mg/L，菌藻共生系统的氨氮去除率最低，为75.27%。通过扫描电镜观察发现，细菌可以通过附着于微藻细胞表面和分泌胞外多聚物来减轻四环素的胁迫效应。通过微生物群落分析表明，四环素质量浓度对细菌群落结构的影响远大于降解菌的接种，降解菌的接种可以减轻低质量浓度四环素对细菌群落结构的影响。优势四环素降解菌Trichococcus减轻了低质量浓度四环素对菌藻共生系统的影响，优势抗逆菌Pseudomonas和反硝化聚磷菌Comamonas减轻了高质量浓度四环素对菌藻共生系统的影响。

关键词: 菌藻共生系统, 四环素, 降解菌, 污染物去除, 微生物群落

Abstract: This study investigated the effects of tetracycline mass concentration and tetracycline degrading bacteriumSerratia entomophilastrain TC-1 on the removal rate of pollutants in the microalgal-bacterial symbiotic system. Through scanning electron microscopy observation and high-throughput sequencing technology, the changes in microbial community structure were further analyzed to reveal the pollutant removal patterns and microbial response mechanisms of the bacterial algal symbiotic system under tetracycline stress. The research results showed that the higher the tetracycline concentration, the lower the microalgal biomass, and the lower the ammonia nitrogen removal rate of the microalgal-bacterial symbiotic system. When the mass concentration of tetracycline was 50 mg/L, the chlorophyll mass concentration was 19.19 mg/L, and the ammonia nitrogen removal rate of the microalgal-bacterial symbiotic system was the lowest, at 75.27%. Through scanning electron microscopy observation, it was found that bacteria could alleviate the stress effect of tetracycline by attaching to the surface of microalgae cells and secreting extracellular polymeric substances. Microbial community analysis showed that the mass concentration of tetracycline had a significantly greater impact on bacterial community structure than the inoculation of degrading bacteria. Inoculating degrading bacteria alleviated the effects of low mass concentrations of tetracycline on bacterial community structure. The tetracycline degrading bacteriaTrichococcusreduced the impact of low mass concentrations of tetracycline on the microalgal bacterial consortia, while the stress resistant bacteriaPseudomonasand denitrifying polyphosphate accumulating bacteriaComamonasreduced the impact of high mass concentrations of tetracycline on the microalgal bacterial consortia.

Key words: microalgal bacterial consortia, tetracycline, degrading bacteria, nurtients removal, microbial community

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