生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (1): 34-.doi: 10.3969/j.issn.2095-1736.2023.01.034

• 研究报告 • 上一篇    下一篇

一株石油降解菌TDYN1基因组测序及分析

陈冠益1,2, 陈红云1, 栗高源1, 胡梦杰3, 李鑫昊1， 钟　磊1   

  1. 1. 天津大学 环境科学与工程学院， 天津 300350； 2. 天津商业大学 机械工程学院，
    天津 300134； 3. 天津商业大学 生物技术与食品科学学院， 天津 300134
  • 出版日期:2023-02-18 发布日期:2023-02-21
  • 通讯作者: 钟磊，博士，副教授，从事环境生态学研究，E-mail: lei.zhong@tju.edu.cn
  • 作者简介:陈冠益，博士，教授，从事农业生物质能源环境与微生物研究，E-mail: chen@tju.edu.cn
  • 基金资助:
    国家重点研发计划政府间国际合作项目(2021yfe0102500)； 天津市自然科学基金项目(19JCQNJC13900)

Genome sequencing and analysis of a highly efficient  oil-degrading bacterium Falsochrobactrum TDYN1

CHEN Guanyi1,2, CHEN Hongyun1, LI Gaoyuan1, HU Mengjie3, LI Xinhao1，ZHONG Lei1   

  1. 1. School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China;
    2. School of Mechanical Engineering, Tianjin University of Commerce, Tianjin 300134, China;
    3. School of Biotechnology and Food Science, Tianjin University, Tianjin 300134, China
  • Online:2023-02-18 Published:2023-02-21

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摘要： 从天津市大港油田附近土壤中分离的新型细菌Falsochrobactrum TDYN1。基于lllumina Hiseq 2000平台对TDYN1进行de novo测序分析，用GO、COG、KEGG、NR以及Swiss-Prot数据库进行基因组基本功能注释。结果表明：TDYN1的基因组测序总长度3 076 677 bp，共得到23个Scaffold，GC含量为55.96%，编码基因共3 465个，其中能够注释到GO、COG、KEGG、NR以及Swiss-Prot的基因数目分别为1 689、2 998、1 946、3 197以及2 513个；同时，预测到TDYN1拥有与石油烃代谢相关的代谢通路，该菌株具有能够降解二英、多环芳烃、硝基甲苯等的能力。通过对TDYN1的全基因组测序和特异性功能基因分析，为进一步对菌株TDYN1的石油烃降解机制及应用研究奠定理论依据，为生产功能菌提供参考数据。

关键词: 石油降解菌, Falsochrobactrum TDYN1, 基因组测序, 基因注释

Abstract: Falsochrobactrum TDYN1 was isolated from the Dagang oilfield in Tianjin. Based on lllumina Hiseq2000, the most widely used second-generation sequencing platform, TDYN1 was de novo sequenced. The basic function of GO, KEGG, COG, NR and Swiss-Prot databases was annotated. The results indicated that the size of the TDYN1 genome was 3076677 bp with 23 Scaffolds, and an average of GC content at 55.96%, and 3465 genes were successfully predicted, which 1689, 1946, 2998, 2513, 2841 and 3197 genes could be matched with annotation information in the GO, KEGG, COG, Swiss-Prot and NR databases respectively. At the same time, it was predicted that TDYN1 had pathways related to petroleum hydrocarbon metabolism, and the ability to degrade dioxins, polycyclic aromatic hydrocarbons and so on. Genome sequencing and analysis of TDYN1 laid an important basis for further research on the mechanism and application of petroleum hydrocarbon degradation of TDYN1, and provided theoretical data for the production of functional bacteria.

Key words: petroleum degrading bacterium, Falsochrobactrum TDYN1, genome sequencing, gene function annotation

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