生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (1): 112-.doi: 10.3969/j.issn.2095-1736.2023.01.112

• 技术方法 • 上一篇    下一篇

比率荧光传感器对硝酸根的快速可视化检测

陶慧慧1， 李玉成1， 张　曌1， 徐诗皓2， 李凌飞2， 曹　巧3   

  1. 1.安徽大学 资源与环境工程学院， 合肥 230601； 2.中国科学院合肥物质科学研究院固体物理所，
    合肥 230031； 3.中国科学院合肥物质科学研究院智能机械所， 合肥 230031
  • 出版日期:2023-02-18 发布日期:2023-02-21
  • 通讯作者: 李玉成，博士，教授，主要研究方向为水土流失控制、生态农业与农业废弃物资源化，E-mail: Li-yucheng@163.com
  • 作者简介:陶慧慧，硕士研究生，从事污染控制与修复研究，E-mail: 1620433034@qq.com
  • 基金资助:
    国家科技重大专项“派河小流域山林地水源涵养技术筛选验证与应用推广”(2017ZX07603-002-001)

Rapid visual detection of nitrate by ratiometric fluorescent sensor

TAO Huihui1, LI Yucheng1, ZHANG Zhao1, XU Shihao2, LI Lingfei2, CAO Qiao3   

  1. 1. School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China;
    2. Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, China;
    3. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031, China
  • Online:2023-02-18 Published:2023-02-21

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摘要： 采用简单、低成本的方法合成一种金纳米团簇（Au NCs）/蓝色碳点（BCDs）体系的比率荧光探针，用于可视化的快速检测亚硝酸根（NO-2）。NO-2可快速且选择性地淬灭Au NCs的红色荧光，BCDs的蓝色荧光作为参考信号不受NO-2的影响。在紫外光激发下，比率荧光探针的荧光颜色随着NO-2的增加从红色变成蓝色。通过研究比率荧光探针的响应时间，该反应体系可以在2 min内完成反应。比率荧光探针的荧光强度比与NO-2浓度在75～200 μmol/L范围内有良好的线性关系，检测限（LOD）低至0.8 μmol/L。以钒离子作为还原剂将NO-3还原为NO-2，还原率达到90%以上，比率荧光探针可以间接检测NO-3浓度，检测限低至0.6 μmol/L。采用该探针对水样和土样测定，加标回收率为94.7%～104.51%。测定结果与分光光度法基本一致，实现了对NO-3的快速、灵敏、准确的检测。基于此，构建了荧光试纸，其展现出对NO-2的高选择性和高灵敏性。荧光试纸不仅提高了检测效率，还降低了检测成本，通过实验证明其快速半定量检测真实样品中NO-3的可行性。

关键词: 金纳米团簇, 比率荧光探针, 硝酸根, 荧光试纸, 可视化检测

Abstract: One Au NCs/blue carbon dots-based ratiometric fluorescent probe, which was synthesized by a simple and low-cost method, was developed to detect NO-2rapidly and visually. The red fluorescence of Au NCs can be quenched by NO-2sensitively and selectively, while the fluorescence of BCDs acting as background reference, remained blue. The probe showed colour changes from red to blue under ultraviolet in response to NO-2. By studying the response time of the ratiometric fluorescence probe, the reaction system could complete the reaction within 2 minutes. The fluorescence intensity ratio of the ratiometric fluorescent probe had a good linear relationship with NO-2concentration in the range of 75-200 μmol/L with a detection limit (LOD) as low as 0.8 μmol/L. NO-3was reduced to NO-2with vanadium ion as reducing agent, and the reduction rate can reach more than 90%. Therefore, NO-3concentration was detected by ratiometric fluorescent probe indirectly, with a detection limit of 0.6 μmol/L. The method was applied to the determination of NO-3in water and soil samples, and the spiked recovery rate was between 94.7%-104.51%. The measured results were basically consistent with the spectrophotometric method, which realized the rapid, sensitive and accurate detection of NO-3. Based on this, a fluorescent test paper has been constructed and it showed high selectivity and sensitivity to NO-2. The fluorescent test paper not only extraordinarily improved the detection efficiency, but also cut down its cost. The feasibility of rapid semi-quantitative detection of NO-3in real samples by fluorescent test paper was proved by experiments.

Key words: AuNCs, ratiometric fluorescent probe, nitrate, fluorescent test paper, visual detection

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