生物学杂志

生物学杂志

• 教学研究 • 上一篇    下一篇

反相纸层析法分离油菜籽中脂肪酸的经验总结

  

  1. 华中农业大学 植物科学技术学院 作物学国家级实验教学示范中心， 武汉 430070
  • 出版日期:2019-04-18 发布日期:2019-04-18
  • 通讯作者: 丰胜求，博士，副教授，从事植物生理学与基础生物化学教学与研究，E-mail: fengsq@mail.hzau.edu.cn
  • 作者简介:李海霞，博士，实验师，从事植物生理生化实验教学与管理， E-mail: lihx2011@mail.hzau.edu.cn
  • 基金资助:
    2015年校级百门精品实践课程《基础生物化学实验》建设项目

Experience summarization in the separation of fatty acids from rapeseed by reversed-phase paper chromatography

  1. National Experimental Teaching Demonstration Centre of Crop Science, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2019-04-18 Published:2019-04-18

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摘要： 在利用反相纸层析法分离油菜籽中主要不饱和脂肪酸的实验教学中，由于受到多种因素的影响，脂肪酸层析图谱经常出现各种问题。经过大量实验摸索，不断优化实验条件，提高了反相纸层析法分离主要不饱和脂肪酸的实验教学效果。对脂肪酸层析进行经验总结，同时对常见问题进行解析并提出相应的解决方案，指导脂肪酸层析实验教学。

关键词: 不饱和脂肪酸, 反相纸层析, 经验总结, 问题解析, 实验教学

Abstract: In the experimental teaching in the separation of main unsaturated fatty acids from rapeseed by reversed-phase paper chromatography, the fatty acid chromatogram often appears various problems due to a variety of factors. Through a lot of experiments and continuous optimization of experimental conditions, the experimental teaching effect of the separation of main unsaturated fatty acids by the reversed-phase paper chromatography was improved. The experience of fatty acid chromatography was summarized. Meanwhile, the common problems were analyzed and the corresponding solutions were putted forward. These can guide the experimental teaching of fatty acid chromatography.

Key words: unsaturated fatty acids, reversed-phase paper chromatography, experience summarization, problem analysis, experimental teaching

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