生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (3): 50-.doi: 10.3969/j.issn.2095-1736.2020.03.050

• 研究报告 • 上一篇    下一篇

长期魔芋低聚甘露糖处理可改善小鼠焦虑样行为

  

  1. 1. 云南师范大学 体育学院 国家级高原训练实验教学中心； 2. 云南师范大学 生命科学学院 生物能源持续开发利用教育部工程研究中心 云南省生物质能与环境生物技术重点实验室, 昆明 650500
  • 出版日期:2020-06-18 发布日期:2020-06-10
  • 通讯作者: 王功伍，博士，副教授，硕士生导师，研究方向为神经生物学，E-mail: waov@tom.com
  • 作者简介:王晓琴，硕士，副教授，研究方向为神经生物学，E-mail: xiaoqinwang2001@163.com
  • 基金资助:
    国家自然科学基金项目（31760278）；教育部“春晖计划”科研项目（Z2011126）；云南师范大学教师教育改革研究项目（2018-8）

Antianxiety effect of long-term treatment of Konjac mannan-oligosaccharides in mice

  1. 1. National Altitude Training Experimental Teaching Demonstrating Center, School of Physical Education;2. Engineering Research Center of Sustainable Development and Utilization of Biomass, Ministry of Education & Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment,School of Life Sciences, Yunnan Normal University, Kunming 650500, China
  • Online:2020-06-18 Published:2020-06-10

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摘要： 魔芋低聚甘露糖(KMOS)是一类重要的益生元，具有多种肠道和机体健康效应，但其中枢神经效应尚不清楚。为探讨KMOS对小鼠情绪相关神经行为及海马神经元密度的影响，将成年雄性昆明小鼠50只，分为5组，给药剂量分别是0（对照组）、650、1400、3000和6500 mg/kg，连续灌胃30 d；然后依次进行旷场、高架十字迷宫和强迫游泳实验，最后高剂量麻醉处死，取脑切片染色，镜检计数海马神经元密度。结果表明，KMOS_6500 mg/kg组腹侧海马CA3亚区神经元密度较对照组增加(P<0.01)，且与KMOS剂量正相关(P<0.01)；旷场实验中，1400 mg/kg组和3000 mg/kg组中央区进入次数和直立次数均高于对照组(均P<0.01)；高架十字迷宫实验中，3000 mg/kg组开臂进入次数高于对照组(P<0.01)。结果提示，长期使用KMOS可促进小鼠腹侧海马CA3区神经元存活，有一定抗焦虑作用。

关键词:  , 魔芋低聚甘露糖；海马；旷场；高架十字迷宫；强迫游泳；昆明小鼠

Abstract: Konjac mannan-oligosaccharides (KMOS) are a kind of important prebiotic. In present study, in order to investigate the effects of KMOS on emotional neural behaviors and the hippocampal neuron density in mice, we divided 50 adult male KM mice into 5 groups， and fed them with KMOS of 0 (control), 650, 1400, 3000 and 6500 mg/kg (i.g.) for consecutive 30 d, and then the mice were tested in the open-field, elevated plus maze (EPM) and forced swimming tasks. At last, their brains were sectioned and stained for hippocampal neuron density counting. Results showed that, the ventral hippocampal CA3 neuron density of KMOS group (6500 mg/kg) increased significantly (P<0.01, vs. control) and positively correlated with the doses (P<0.01). In open field, the rearing times and center entries of KMOS groups (1400 and 3000 mg/kg) significantly increased (P<0.01, vs. control). In EPM, the open arms entries of mice in 3000 mg/kg group also increased (P<0.01, vs. control). The results suggested that long-term treatment of mice in KMOS would be involved in neuron survival in hippocampal CA3 subarea and have anxiolytic effect in mice.

Key words: Konjac mannan-oligosaccharides, hippocampus, open field, elevated plus maze, forced swimming, KM mice

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