生物学杂志 ›› 2020, Vol. 37 ›› Issue (3): 21-.doi: 0.3969/j.issn.2095-1736.2020.03.021

• 研究报告 • 上一篇    下一篇

水稻稻瘟病抗性基因Pik-2的遗传变异及进化分析

  

  1. 1. 湖南农业大学 农学院, 长沙 410128; 2. 杂交水稻国家重点实验室湖南省杂交水稻研究中心, 长沙 410125; 3. 国际水稻研究所, 菲律宾 马尼拉 0900
  • 出版日期:2020-06-18 发布日期:2020-06-10
  • 通讯作者: 肖应辉,研究员,主要从事水稻遗传育种研究,E-mail: xiaoyh@hunau.edu.cn;周波,研究员,主要从事水稻抗病机制研究,E-mail:irri2014@163.com
  • 作者简介:牛玉芳,硕士研究生,研究方向为种子生物学,E-mail:yufang1018@163.com
  • 基金资助:
    湖南省自然科学基金(2018JJ3380 );湖南省农业科学院科技创新项目(2017JC08)

Genetic variation and evolution of Pik-2, a resistance gene against rice blast

  1. 1. Agronomy College, Hunan Agricultural University, Changsha 410128, China;2. Hunan Hybrid Rice Research Center, State Key Laboratory of Hybrid Rice, Changsha 410125, China;3. International Rice Research Institute, Makati City 0900, Philippines
  • Online:2020-06-18 Published:2020-06-10

摘要: Pik位点是水稻第11染色体上稻瘟病抗性基因成簇分布的热点区域,对该位点抗性基因在水稻品种中多样性分布的研究,有助于揭示该基因的进化规律和进一步挖掘其等位基因。对来自不同国家和地区的400份非洲稻和400份亚洲稻稻种抗稻瘟病基因Pik-2 编码区序列进行了测序分析,发现非洲稻和亚洲稻品种Pik-2 编码区碱基变异非常丰富,非洲稻种中出现了113个变异位点,亚洲稻种中有78个碱基位点发生多样性变异,其中两者均在1879位点显示出极高的变异频率;根据其碱基变异特点,非洲稻种和亚洲稻种分别呈现出17和12种单倍型。单倍型的地区分布研究显示,非洲稻的分布呈现出大聚集、小分散的特点,而亚洲稻的分布来源就显得非常广泛。不同单倍型的系统进化分析表明,大多类群中同时包含非洲稻与亚洲稻的单倍型,暗示非洲稻与亚洲稻在相互独立的进化过程中在Pik-2 LRR结构域各自形成了相似的单倍型。

关键词: 非洲稻;亚洲稻;稻瘟病;抗病基因, Pik-2

Abstract: The Pik locus, located on the long arm of chromosome 11, is a hotspot region for rice blast resistance. To study the diversity and distribution of the resistance (R) genes at the Pik locus will be useful for revealing the evolutionary mechanisms of this gene and to further explore its alleles. We sequenced and analyzed the Pik-2 coding region of 400 Oryza glaberrima and 400 Oryza sativa cultivars from different countries and regions. 113 and 78 polymorphic sites were found in O. glaberrima and O. sativa, respectively, which showed high mutation frequency at the nucleotide position 1879. According to the characteristics of the base variations, 17 haplotypes of O. glaberrima and 12 haplotypes of O. sativa were identified. The results indicated that the Pik-2 haplotype distribution in O. glaberrima was characterized by large aggregation and small dispersion, in contrast to the very extensive distribution in O. sativa. Phylogenetic analysis of different haplotypes showed that most groups contained both African and Asian rice haplotypes, suggesting that African and Asian rice had similar haplotypes in the Pik-2 LRR domain during their independent evolution.

Key words: Oryza glaberrima, Oryza sativa, rice blast, resistance gene, Pik-2

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