生物学杂志

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玉米秸秆水抽提物对乙醇发酵的影响

  

  1. 1. 南京林业大学化学工程学院,江苏 南京 210037;2. 江苏省生物质绿色燃料与化学品重点实验室,江苏 南京 210037
  • 出版日期:2016-02-18 发布日期:2016-02-18
  • 通讯作者: 朱均均,副教授,博士,研究领域为生物化工,E-mail: zhujj@njfu.edu.cn

Effect of water extractives from corn stover on ethanol fermentation

  1. 1.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing 210037, China
  • Online:2016-02-18 Published:2016-02-18

摘要:  

采用水抽提方法提取玉米秸秆中的水抽提物,研究水抽提液、水抽提液稀酸水解液对树干毕赤酵母木糖乙醇发酵和酿酒酵母葡萄糖乙醇发酵性能的影响。研究结果表明,与空白对照相比,添加水抽提液对树干毕赤酵母木糖乙醇发酵性能有一定的抑制作用,随着水抽提液添加体积的增加,抑制程度有所增加,但增加不明显,延长时间至48 h可以增加糖利用率,但乙醇得率均呈现下降趋势(80 %以下);而添加水抽提液对酿酒酵母葡萄糖乙醇发酵的抑制作用不明显。当添加水抽提液稀酸水解液时,树干毕赤酵母木糖乙醇发酵时间延长(从24 h延长到48 h),达到相同糖利用率时的乙醇得率下降;而添加水抽提液稀酸水解液对酿酒酵母葡萄糖乙醇发酵性能抑制作用不大。因此,树干毕赤酵母要比酿酒酵母对水抽提液及其稀酸水解液中存在的抑制物更敏感,所以在玉米秸秆预处理之前,采用水抽提去除水抽提物可减少水抽提物在后续预处理过程中产生抑制物,从而提高微生物乙醇发酵能力,为燃料乙醇真正商业化生产提供参考依据。

关键词:  ,

Abstract:   

Water extractives were obtained by water extraction of corn stover. The effects of water extractives or its diluted acid hydrolyzate from corn stover on xylose fermentation to ethanol by Pichia stipitis and glucose fermentation to ethanol by Saccharomyces cerevisiae were investigated. The results showed that, compared to the control, there were some negative effect on xylose fermentation to ethanol by P. stipitis when the water extractives were added to the medium. The degree of inhibition increased with the increase of water extractives, but not obvious. The sugar utilization ratio increased when the fermentation time increased to 48 h, however, the ethanol yield decreased (less than 80 %). There was little negative effect on glucose fermentation to ethanol by S. cerevisiae when the water extractives were added to the medium. When the diluted acid hydrolyzate of water extractives was added to the medium, the fermentation time of xylose to ethanol by P. stipitis extended from 24 h to 48 h, and the ethanol yield decreased while the sugar utilization ratio was the same. However, the diluted acid hydrolyzate of water extractives still had little negative effect on glucose fermentation to ethanol by S. cerevisiae. Therefore, P. stipitis was more sensitive than S. cerevisiae to the inhibitors existed in the water extractives and its diluted acid hydrolyzate. Water extraction before corn stover pretreatment could reduce the inhibitors produce; thereby improved the ethanol fermentability by yeasts. Finally, it provides reference for real commercial production of fuel ethanol.