生物学杂志

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拟南芥生物钟突变体gi延迟营养生长时相转变

  

  1. 天津农学院 园艺园林学院, 天津 300384
  • 出版日期:2018-06-18 发布日期:2018-06-18
  • 通讯作者: 龙鸿,博士,教授,从事植物发育生物学研究,E-mail: longhong@mail.hzau.edu.cn
  • 作者简介:郭蕊,硕士,研究生,专业方向为果树生物技术,E-mail:961286947@qq.com
  • 基金资助:
    教育部留学回国人员基金项目(No.4002-082017)

The vegetative phase change of circadian clock mutant gi in Arabidopsis thaliana#br#  

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China
  • Online:2018-06-18 Published:2018-06-18

摘要: 高等植物营养生长时相转变是植物生长发育的一个重要生理过程,与植物自身生物钟节律密切相关,但生物钟相关基因在此转变中的作用仍不明确。研究了拟南芥(Arabidopsis thaliana)生物钟突变体gi的营养生长时相转变特点。结果表明,gi的生长周期比野生型的延长,从莲座叶叶片形态、叶片长宽比、远轴面表皮毛出现等营养生长形态特征和茎尖分生组织解剖结构特征分析,gi突变体延迟了营养生长时相转变。此外,RT-PCR和Q-PCR检测营养生长时相转变过程中起关键作用的miR156的表达量结果也显示,gi突变体延迟了营养生长时相转变。突变体由于生长的生物钟受到影响,改变了生长的节律,茎尖分生组织中原套产生多层细胞较晚,原体和髓分生组织的细胞发育延迟,暗示GI可能通过影响茎尖分生组织发育来调控营养生长时相转变。

关键词: 营养生长时相转变, 生物钟, gi, 拟南芥

Abstract: VPC (vegetative phase change) of higher plants undergo two stages, juvenile and adult phase. VPC is one of the important physiological processes in plant development, which is related to circadian clock regulation. Unfortunately, circadian clock related genes regulation during this transition remains unknown. In this paper, we investigated characters of VPC in a mutant of circadian clock-related gene, GI (Gigantea). Results showed that the life span of gi was prolonged. Onset of abaxial trichomes of rosette leaves, leaf shape, the length-to-width ratio of the lamina, and paraffin section of shoot apical meristems indicated that vegetative phase change in gi was delayed. Moreover, the RT-PCR and q-PCR of miR156, a key molecular factor regulating VPC, showed equivalent consequence with morphological and anatomical analysis. Compared with wild plants, multi-layers of tunica cells were onset later, corpus and pith meristem zone developed later in mutant plants, due to the changed growth rhythms controlled by the circadian clock. Our data suggested that GI might play a role in regulating plant VPC through affecting the development of SAM.

Key words: vegetative phase change, circadian clock, gi, Arabidopsis thaliana