生物学杂志

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马尾藻提取物对亚硝化反应的抑制作用

  

  1. 北京师范大学珠海分校 工程技术学院, 珠海 519085
  • 出版日期:2018-06-18 发布日期:2018-06-18
  • 通讯作者: 汪波,副教授,研究方向为生物资源开发与利用,E-mail:wangbozai@163.com
  • 作者简介:李秀菊,专业方向为生物资源利用,E-mail:stellalxj@qq.com
  • 基金资助:
    广东省“攀登计划”专项资金项目;省级大学生创新训练项目(№:201613177034)

Inhibitory effect of sargassum extract on nitrosation reaction

  1. School of Engineering Technology, Beijing Normal University, Zhuhai Campus, Zhuhai 519085, China
  • Online:2018-06-18 Published:2018-06-18

摘要: 基于马尾藻安全性检测,用醇提法对马尾藻进行提取,探究不同浓度乙醇提取物对亚硝胺合成的阻断作用以及清除亚硝酸盐的能力。重金属含量检测方法参考电感耦合等离子体质谱法,农药残留检测方法参考气相色谱-质谱法;用浓度为0%、25%、50%、75%和100%的乙醇分别对马尾藻进行提取,采用α-萘胺法测定提取物对亚硝胺合成的阻断作用,采用盐酸萘乙二胺比色法测定提取物清除亚硝酸盐的能力。马尾藻重金属含量均在安全范围内,各项农药残留均未检出;乙醇浓度为50%和75%时,马尾藻提取物对亚硝胺合成的阻断率达100%;乙醇浓度为75%时,马尾藻提取物清除亚硝酸盐的能力最佳,清除率为53.33%。马尾藻提取物能有效阻断亚硝胺合成和清除亚硝酸盐,可应用于开发新的防癌抗癌天然保健品。

关键词: 马尾藻, 安全性检测, 亚硝胺, 亚硝酸盐

Abstract: Based on the safety of Hizikia fusiformis, the H. fusiformis extracted by ethanol was used to investigate the blocking effect of different concentrations of ethanol extracts on the synthesis of nitrosamine and the ability to remove nitrite. The detection of heavy metals referenced inductively coupled plasma mass spectrometry, and the pesticide residue detection method referenced gas chromatography mass spectrometry; the H. fusiformis was extracted with 0, 25%, 50%, 75% and 100% ethanol, respectively. The blocking effect of the extract on the synthesis of nitrosamines was determined by α-naphthylamine method.The ability of the extract to remove nitrite was determined by the color reaction of naphthalene ethylenediamine hydrochloride. The content of heavy metals in H. fusiformis was within the safe range, and the pesticide residues were not detected. When the ethanol concentrations were 50% and 75%, the blocking rate of the synthesis of nitrosamine was 100%; when the ethanol concentration was 75%, H. fusiformis extract had the best ability to remove nitrite with the removal rate of 53.33%. H. fusiformisdid not have pesticide residues, which extract could effectively block the synthesis of nitrosamines and removed nitrite and can be used to develop new anti-cancernatural healthproducts.

Key words: Hizikia fusiformis, safety test, nitrosamines, nitrite