生物学杂志

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藻际芽孢杆菌对坛紫菜生理的影响

  

  1. 宁波大学 海洋学院 浙江省海洋生物工程重点实验室, 宁波 315211
  • 出版日期:2018-02-18 发布日期:2018-02-18
  • 通讯作者: 杨 锐,副研究员,研究方向为藻类生物技术,E-mail:yangrui@nbu.edu.cn
  • 作者简介:熊玉琴,硕士研究生,研究方向为藻际生物技术,E-mail:xiongyuqin223@163.com
  • 基金资助:
    国家级星火计划项目(2013GA701001);国家“111”计划;浙江省水产养殖重点学科发展基金(#xkzsc1509)

Effects of phycosphere Bacillus sp. WPySW2 on physiology of Pyropia haitanensis

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science,Ningbo University, Ningbo 315211, China
  • Online:2018-02-18 Published:2018-02-18

摘要: 以宁波象山坛紫菜(Pyropia haitanensis)叶状体和紫菜附生菌——芽孢杆菌(Bacillus sp. WPySW2)为材料,研究在正常培养温度(20℃)下,不同时间( 0、6、12、24、48和72 h) 藻-菌共养处理后附生菌对坛紫菜叶状体中过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、光合色素、总蛋白、游离脯氨酸(Pro)和丙二醛(MDA)含量的影响。结果发现:适宜温度下,与坛紫菜纯培养系统(Ph-C)相比,藻-菌共养系统(Ph-B)有利于藻体光合色素和蛋白含量的积累;Ph-B组和Ph-C组细胞膜均未受到氧化,即抗氧化酶含量无显著性差异(P>0.05);两组都产生过量膜脂过氧化物和渗透压调节物质,但是Ph-C组产生得更多(P<0.05)。由此推测,藻际附生菌可能为藻际微环境中的藻体提供营养物质来影响其生长状态及营养物质的积累。

关键词: 坛紫菜, 芽孢杆菌, 藻际微环境

Abstract: Pyropia haitanensis collected from the culture area of the Xiangshan, Zhejiang Province, was pure cultured and co-cultured with Bacillus sp. WPySW2 from Porphyra-epiphytic bacteria under different time (0, 6, 12, 24, 48 and 72 h). They grew at the normal culture temperature (20℃), and the physiology were measured, including the activities of (POD), superoxide dismutase (SOD), and also photosynthetic pigments, total protein, free proline and malondialdehyde (MDA) levels. The results showed that the content of photosynthetic pigments and protein in co-culture group was better than that in the control. Both were no significant difference in antioxidant enzyme activity because of cell membranes been not oxidized (P>0.05). The two groups produced excessive membrane lipid peroxidation and osmotic pressure regulating substance, but the control group produced more (P<0.05). It was speculated that the phycosphere epiphytic bacteria would provide nutrients for the algae in phycosphere to affect their growth status and the accumulation of nutrients.

Key words: Pyropia haitanensis, Bacillus sp. WPySW2, phycosphere