生物学杂志

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不同年生凤丹根际AM真菌与土壤酶活性的相关性分析#br#

  

  1. 安徽师范大学 生命科学学院 生物环境与生态安全安徽省高校省级重点实验室, 芜湖 241000
  • 出版日期:2018-02-18 发布日期:2018-02-18
  • 通讯作者: 杨安娜,博士,研究方向为菌根生物学,E-mail:anyz@163.com
  • 作者简介:志雅,硕士研究生,研究方向为菌根生物学,E-mail:2370905644@qq.com
  • 基金资助:
    国家自然科学基金(31600026);安徽省高校自然科学研究重点项目(KJ2016A276);安徽师范大学博士启动基金项目(2014bsqdjj50)

The correlation analysis of soil enzyme activity and AM fungi in the rhizosphere soil of different year-old Paeonia ostii

  1. Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui, College of Life Sciences,Anhui Normal University, Wuhu 241000, China
  • Online:2018-02-18 Published:2018-02-18

摘要: 利用碱解离-酸性品红方法测定凤丹根样AM真菌结构定殖情况,同时进行土壤菌丝含量、土壤孢子密度及土壤酶活性的测定。结果表明,全部根样均被AM真菌侵染,并形成了典型Arum-type类型的AM结构;AM真菌定殖率、菌丝定殖率、丹皮酚含量及孢子密度在不同年生呈现出相同趋势,均表现为3年生凤丹<4年生凤丹<2年生凤丹,而泡囊定殖率与菌丝圈定殖率呈现出相同趋势,表现为3年生凤丹<2年生凤丹<4年生凤丹;酸性磷酸酶、脲酶、蔗糖酶、多酚氧化酶的活性与土壤菌丝含量呈正相关,酸性磷酸酶、脲酶、蔗糖酶、多酚氧化酶的活性与泡囊定殖率呈负相关,孢子密度和菌丝圈定殖率与酸性磷酸酶、蔗糖酶、多酚氧化酶的活性呈正相关,与脲酶活性呈负相关;菌丝定殖率、AM真菌定殖率与酸性磷酸酶的活性呈正相关,与脲酶、蔗糖酶、多酚氧化酶的活性呈负相关。揭示了在休眠期,不同年生(2、3、4年生)的凤丹根际AM真菌(arbuscular mycorrhizae fungi,AM真菌)共生状况、根部丹皮酚含量测定及土壤酶活性变化。

关键词: 凤丹, 丛枝菌根真菌, 定殖率, 土壤酶活性, 丹皮酚, 孢子密度, 土壤菌丝含量

Abstract: The method of alkaline hydrolys acid fuchsin staining was used for determining the colonization of AM fungi in the root of Paeonia ostii. The content of soil hypha, spore density in soil and the activity of soil enzyme were also determined. The results showed that all roots samples were infected by AM fungi and formed the structure of Arum-type AM. AM fungal colonization rate in Triennial Paeonia ostii is smaller than the four-year-old Paeonia ostii′s which was smaller than the Biennial Paeonia ostii′s. Hypha colonization rate, content of paeonol and spore density in different years′ root samples showed the same trend as the AM fungal colonization rate. The colonization of vesicle in Triennial Paeonia ostii was smaller than that in Biennial Paeonia ostii′s, the biggest one was four-year-old Paeonia ostii′s. The colonization of hyphal coil showed the same trend as the colonization of vesicle.The activity of acid phosphatase, urease, sucrase and polyphenol oxidase was positively correlated with the content of soil hypha and negatively correlated with the colonization of Vesicle. The activity of acid phosphatase, sucrase and polyphenol oxidase was positively correlated with the colonization of hyphae circle and spore density, but negatively correlated with the urease activity. The activity of acid phosphatase was positively correlated with the colonization of hypha and AM fungi, but negatively correlated with the urease, sucrase and polyphenol oxidase′s activity. The research reveals the symbiotic relationship of AM fungi with the different year-old root of Paeonia ostii during period of dormancy and determinates the content of cortex moutan phenol and the activity of soil enzyme.

Key words: Paeonia ostii, arbuscular mycorrhizal fungi, colonization rate, soil enzyme activity, cortex moutan phenol, spore density, the content of soil hypha