生物学杂志

• 研究报告 • 上一篇    下一篇

酸枣内生菌的筛选及其次级代谢产物的初步分离

  

  1. 延安大学 生命科学学院, 延安 716000
  • 出版日期:2018-02-18 发布日期:2018-02-18
  • 作者简介:邓振山,博士,副教授,主要从事植物内生菌资源开发和利用及环境微生物研究,E-mail:zhenshandeng214@163.com
  • 基金资助:
    国家级大学生创新训练计划项目( 201310719007);陕西省科技统筹创新工程项目(2012KTZB03-02-03); 陕西省科技统筹创新工程项目(2012CGX7)

Screening of endophytes in Zizyphus jujube and the analysis of their secondary metabolites

  1.  School of Life Science, Yan′an University, Yan′an 716000, China
  • Online:2018-02-18 Published:2018-02-18

摘要: 充分开发利用酸枣资源,寻找生产黄酮类化合物等活性物质的内生菌,为新型药物提供种质资源,采用组织分离法、划线分离法分离纯化酸枣茎的内生菌,并用平板对峙法对抑菌活性进行测定。抗菌活性较强的菌株有B-08-12和B-08-20,抑菌圈直径达到(31.00±0.78) mm;并将菌株进行发酵,用有机试剂萃取、薄层层析以及紫外分光光度计作进一步检测。薄层层析检测表明:B-08-20菌株的发酵产物在Rf为0.43处有与酸枣茎提取液斑点Rf值相同的显色斑点,初步确定活性物质的种类为黄酮类或者其类似物。

关键词: 酸枣, 内生菌, 拮抗性, 薄层层析, 黄酮类

Abstract: To fully exploit and utilize the resources of wild jujube, look for endophytes producing active substances such as flavonoids, provide germplasm resources for new drugs, isolate and purify the endophytes of the jujube stem by tissue separation method and sutural separation method, confrontation method for antibacterial activity was measured. The strains with strong antibacterial activity were B-08-12 and B-08-20, the diameter of the zone of inhibition was (31.00±0.78) mm, and the strains were fermented, extracted with organic reagents, investigated by thin layer chromatography (TLC) and also analyzed by UV spectroscopy Photometer for further detection. TLC detection showed that the fermentation product of strain B-08-20 had the same color speckles at the Rf of 0.43 as the Rf value of the jujube stem extract, and the active compounds were preliminarily identified as flavonoids or its analogues.

Key words: Ziziphus jujube, endophyte, antibacterial activity, TLC, flavonoids