生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (5): 27-.doi: 10.3969/j.issn.2095-1736.2026.05.027

• 研究报告 • 上一篇    下一篇

烟曲霉聚半乳糖醛酸酶的酶学特性及其水解落叶应用

钟　璇1， 王青艳2， 吴晶桃2， 郑孟婷2， 秦　艳2， 冼　亮2   

  1. 1. 广西职业技术学院 农业工程学院， 南宁 530226； 2. 广西科学院 非粮生物质能技术全国
    重点实验室 国家非粮生物质能源工程技术研究中心， 南宁 530007
  • 出版日期:2026-10-18 发布日期:2026-10-20
  • 通讯作者: 秦艳，副研究员，研究方向为发酵工程，E-mail：307130339@qq.com；冼亮，副研究员，研究方向为食品用酶相关技术，E-mail：liangxianpublish@163.com；秦艳和冼亮为共同通信作者
  • 作者简介:钟璇，硕士，教授，研究方向为食品精深加工及酶制剂应用，E-mail：710998952@qq.com
  • 基金资助:
    国家自然科学基金项目（32360560）； 中央引导地方科技发展专项（桂科ZY23055011）； 广西重点研发计划项目（桂科AB24010349）

Enzymatic characteristics of polygalacturonase produced by an Aspergillus fumigatus strain and its application in fallen-leaf hydrolysis#br#

ZHONG Xuan1, WANG Qingyan2, WU Jingtao2, ZHENG Mengting2, QIN Yan2, XIAN Liang2#br#   

  1. 1. School of Agricultural Engineering, Guangxi Vocational and Technical College, Nanning 530226, China;
    2. National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food
    Biomass Energy Technology, Guangxi Academy of Sciences, Nanning 530007, China
  • Online:2026-10-18 Published:2026-10-20

PDF (PC)

0

摘要： 寻找适合落叶水解的聚半乳糖醛酸酶对促进自然界物质循环至关重要。通过采集特定样品增加针对性，测定酶学特性检测聚半乳糖醛酸酶的适配性，通过落叶水解实验衡量所获微生物的应用潜力。从腐烂落叶中分离筛选得到一株聚半乳糖醛酸酶产生菌F21-5，并通过形态学特征和内转录间隔区编码序列分析鉴定为烟曲霉。F21-5液体培养产生的粗聚半乳糖醛酸酶最适作用pH为5.5，在pH 4.0~6.0能发挥出的酶活力达到其最高值的80%以上，并且其在pH 3.0~11.0稳定。F21-5粗聚半乳糖醛酸酶的最适作用温度为60~65 ℃，并且其在40 ℃比较稳定。F21-5粗聚半乳糖醛酸酶的活力受Ca2+和Cu2+抑制。F21-5粗聚半乳糖醛酸酶水解白玉兰、柚木、波罗蜜和人面子落叶后将水解体系中还原糖的量分别提升了503.47%±37.26%、416.07%±0.48%、227.03%±0.46%和105.60%±0.27%。本研究发现F21-5粗聚半乳糖醛酸酶表现出优良的酶学特性，并对多种落叶表现出优秀的水解能力，因而烟曲霉或许在自然界的物质循环中发挥重要作用，其聚半乳糖醛酸酶在果胶水解的相关领域有潜在的应用价值。

关键词: 聚半乳糖醛酸酶, 落叶水解, 烟曲霉, 菌株分离和鉴定, 酶学特性

Abstract: The search for a polygalacturonase suitable for fallen leaf hydrolysis is crucial for accelerating the natural nutrient cycle. This study involved collecting corresponding samples to increase the possibility of obtaining target microorganisms, determining enzymatic characteristics to assess the adaptability of polygalacturonase, and hydrolyzing fallen leaves to measure the application potential of the isolated strain. In the study, a polygalacturonase-producing strain, F21-5, was isolated from decaying fallen leaves and identified asAspergillus fumigatusbased on morphological characteristics and internal transcribed spacer sequence analysis. The obtained F21-5 crude polygalacturonase showed an optimal pH of 5.5, retained more than 80% of its activity within the pH range of 4.0－6.0, and remained stable across pH 3.0－11.0. Furthermore, the enzyme exhibited maximum activity at 60－65 ℃ and maintained stability at 40 ℃. The activity of F21-5 crude polygalacturonase was markedly inhibited by Ca2+ and Cu2+ at the tested concentrations. Hydrolysis of fallen leaves increased the reducing sugar concentration by 503.47%±37.26% in leaves ofMagnolia denudata, 416.07%±0.48% inTectona grandisL.f., 227.03%±0.46% inArtocarpus heterophyllusLam., and 105.60%±0.27% inDracontomelon dao. These results demonstrate thatA. fumigatusF21-5 produces a robust polygalacturonase with strong hydrolytic activity, indicating its potential role in leaf degradation and natural nutrient cycling. Furthermore, polygalacturonase produced byA. fumigatusF21-5 represents a high-potential biocatalyst for industrial-scale pectin bioconversion applications.

Key words: polygalacturonase, fallen-leaf hydrolysis;Aspergillus fumigatus, strain isolation and identification, enzymatic characteristics

中图分类号: