生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (1): 60-.doi: 10.3969/j.issn.2095-1736.2026.01.060

• 研究报告 • 上一篇    下一篇

毛果杜鹃根原基发育中内源激素及转录组表达

江　琰， 龚　娜， 林乙明， 李卓群， 冯丽贞， 陈世品   

  1. 福建农林大学 林学院， 福州 350002
  • 出版日期:2026-02-18 发布日期:2026-02-27
  • 通讯作者: 陈世品，博士，教授，主要从事森林培育方面的研究，E-mail：fjcsp@126.com
  • 作者简介:江琰，硕士研究生,主要从事林业方面的研究，E-mail：jyan2023@126.com
  • 基金资助:
    福建农林大学林学高峰学科建设项目（72202200205）；福建省轻纺（控股）有限责任公司科技项目（KH200236A）

Endogenous hormone and transcriptome expression during root primordia development in Rhododendron seniavinii

JIANG Yan, GONG Na, LIN Yiming, LI Zhuoqun, FENG Lizhen, CHEN Shipin   

  1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2026-02-18 Published:2026-02-27

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摘要： 本研究以毛果杜鹃组培苗为材料，采用蒽酮比色法、考马斯亮蓝G-250染色法以及酶联免疫吸附法分别测定可溶性糖含量、可溶性蛋白含量以及生长素（IAA）、赤霉素（GA3）和玉米素核苷（ZR）的含量。结果表明：毛果杜鹃茎内并未观察到潜伏根原基，推定其属诱生型根原基。组培苗基部因细胞分裂和代谢能力增强，可溶性糖含量呈下降趋势，而其中可溶性蛋白的变化规律则与其相反。在探究内源激素对根原基形成的作用机理时，观察到IAA、GA、ZR三者的含量变化呈现出一致的动向趋势，与根原基的形成之间存在较显著的正相关性。在毛果杜鹃根原基形成发育的过程中，RS.ARF12、RS.ARF16、RS.ARF18、RS.ARF4基因贡献出较高的表达量，可以预测它们在根原基形成过程中起到了较为重要的调控作用。

关键词: 毛果杜鹃, 根原基, 石蜡切片, 内源激素, ARF基因家族

Abstract: This study used tissue-cultured seedlings ofRhododendron seniaviniias the experimental material. The anthrone colorimetry, Kaomas Brilliant Blue G-250 staining and enzyme-linked immunosorbent assay （ELISA） were used to determine the content of soluble sugars, soluble proteins, and the content of growth hormone （IAA）, gibberellic acid （GA3）, and zearalenin riboside （ZR）, respectively. The results showed that no latent root primordia were observed in the stems ofR. seniavinii, suggesting that it is an induced root primordia species. At the base of the tissue culture seedlings, soluble sugar content decreased, due to enhanced cell division and metabolic activity. In contrast, soluble protein content increased. Investigation of the role of endogenous hormones in root primordia formation revealed that changes in the contents of IAA, GA, and ZR followed consistent trends and showed a significant positive correlation with root primordia formation. In the process ofR. seniaviniiroot primordia formation, RS.ARF12, RS.ARF16, RS.ARF18, and RS.ARF4 genes were expressed at higher levels, suggesting their important roles in root primordia formation process.

Key words: Rhododendron seniavinii, root primordia, paraffin section, endogenous hormones, ARF gene family

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