生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 24-.doi: 10.3969/j.issn.2095-1736.2025.01.024

• 青藏高原植物资源研究与利用专题 • 上一篇    下一篇

绵参适应高海拔极端环境的转录调控模式研究

赵　宁1,2， 韦亦泠1,3， 刘　星4， 丁亚丽5   

  1. 1. 西藏大学 生态环境学院， 拉萨 850000； 2. 青藏高原生物多样性与生态环境保护教育部重点实验室，
    拉萨 850000； 3. 黔南民族医学高等专科学校 医学系， 都匀 558000； 4. 武汉大学 生命科学学院，
    武汉 430000； 5. 西藏大学 医学院， 拉萨 850000
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 丁亚丽，高级实验师，研究方向为药用植物开发研究，E-mail:1057827768@qq.com
  • 作者简介:赵宁，硕士，讲师，研究方向为青藏高原生物多样性与分子进化，E-mail:982187126@qq.com；韦亦泠，硕士，讲师，研究方向为青藏高原生物多样性与分子进化，E-mail:1922353298@qq.com；赵宁和韦亦泠为共同第一作者
  • 基金资助:
    西藏自治区科技计划中央引导地方项目（XZ202001YD0028C）； 西藏大学校级培育项目成长计划项目(ZDCZJH21-03)

Study of the transcriptional regulatory patterns in Eriophyton wallichii for adaptation to high-altitude extreme environments

ZHAO Ning1,2, WEI Yiling1,3, LIU Xing4, DING Yali5   

  1. 1. School of Ecology and Environment, Tibet University, Lhasa 850000, China; 2. Key Laboratory of Biodiversity
    and Environment on the Qinghai-Tibet Plateau, Ministry of Education, Lhasa 850000, China; 3. Medical Department,
    Qiannan National Medical College, Duyun 558000, China; 4. College of Life Science, Wuhan University,
    Wuhan 430000, China; 5. College of Medicine, Tibet University, Lhasa 850000, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 利用转录组和代谢组综合分析不同海拔绵参的差异代谢物和差异表达基因，筛选出参与绵参适应高海拔极端流石滩环境的关键代谢物及基因，解析绵参适应青藏高原极端流石滩环境的适应性分子基础。结果表明：参与高海拔适应相关的次级代谢物（黄酮类、苯丙素类的生物合成），氨基酸（色氨酸、酪氨酸、苯丙氨酸），脂质（不饱和脂肪酸、甘油磷脂、亚油酸）等在绵参总代谢物中被注释到，并且高海拔居群叶片中黄酮类、苯丙素类等抗氧化类代谢物及相关通路被显著富集；叶片中山柰酚-7-O-葡萄糖苷、山柰酚-7-新橘皮糖苷、芹甙元-7-咖啡基葡萄糖苷和山柰酚-3-葡萄糖苷等物质的积累或与绵参绒毛形成有关；高海拔居群根中谷胱甘肽及抗坏血酸合成相关基因被显著富集，这些基因在抗氧化防御系统中起关键作用。研究为揭示绵参适应高海拔极端环境的机制提供参考。

关键词: 绵参, 转录组学和代谢组学, 高海拔, 适应性, 青藏高原

Abstract: This study integrated transcriptomic and metabolomic analyses to investigate the differential metabolites and gene expression profiles ofEriophyton wallichiiat varying altitudes, aiming to elucidate its adaptive mechanisms to the extreme cobble environment of the Qinghai-Tibet Plateau. Key metabolites and genes associated withE.wallichii’s adaptation to high-altitude conditions were identified. Our results revealed that the total metabolite profiles ofE. wallichiiincluded secondary metabolites (e.g., flavonoids, phenylpropanoids), amino acids (e.g., tryptophan, tyrosine, phenylalanine), lipids (e.g., unsaturated fatty acids, glycerophospholipids, linolenic acid), and other compounds. Flavonoids, phenylpropanoids, and related pathways involved in high-altitude adaptation were notably enriched in the leaves of high-altitude populations. Accumulation of substances such as kaempferol-7-O-glucoside, kaempferol-7-neohesperidoside, Apigenin-7-caffeoylglucoside, and kaempferol-3-glucoside in the leaves was likely linked to the development of trichomes inE.wallichii.Additionally, genes related to glutathione and ascorbic acid synthesis were significantly enriched in the roots of high-altitude populations, playing a crucial role in the antioxidant defense system. This study offered valuable insights into the adaptive mechanisms ofE.wallichiiin high-altitude environments.

Key words: Eriophyton wallichii, transcriptomics and metabolomics, high altitude, adaptation, Qinghai-Tibet Plateau

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