生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 36-.doi: 10.3969/j.issn.2095-1736.2025.01.036

• 研究报告 • 上一篇    下一篇

假单胞菌1616X1的产酸特性及其全基因组测序分析

翟　梅， 李思思， 赵　帅， 黄　峰， 周　佳， 赵雷真， 屈建航   

  1. 河南工业大学 生物工程学院， 郑州 450001
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 屈建航，博士，教授，研究方向为环境微生物学，E-mail:qjh_bata@163.com
  • 作者简介:翟梅，硕士，研究方向为环境微生物学，E-mail:3021838041@qq.com
  • 基金资助:
    河南工业大学创新基金支持计划专项（2021ZKCJ15）； 国家自然科学青年基金项目（42107139）； 郑州市R&D专项经费补助科研项目（22ZZRDZX32）

Acid production characteristics and complete genome sequencing analysis of Pseudomonas sp. 1616X1

ZHAI Mei, LI Sisi, ZHAO Shuai, HUANG Feng, ZHOU Jia, ZHAO Leizhen, QU Jianhang   

  1. School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 为探究假单胞菌溶解难溶性无机磷的作用机理，以高效溶磷假单胞菌1616X1为对象，以Ca3(PO4)2为磷源，高效液相色谱法测定菌株溶磷过程中的有机酸种类及含量变化，通过Illumina HiSeq X Ten测序平台对假单胞菌1616X1全基因组进行测序，序列拼装后完成基因预测、功能注释和溶磷相关基因分析。结果表明，假单胞菌1616X1在溶磷过程中产生葡萄糖酸、酒石酸与柠檬酸等多种有机酸，其中，以葡萄糖酸为主，在溶磷量最高时葡萄糖酸含量达17582.06 mg/L；全基因组测序及分析结果表明，菌株1616X1基因组长度5957620 bp，GC含量61.55%，含有5535个编码基因。经COG、GO和KEGG数据库比对和注释，其中，含有吡咯喹啉醌生物合成基因簇（pqq）、磷酸盐转运系统合成基因（pst与pit）、磷饥饿反应调控基因（phoB、phoR与phoH）以及葡萄糖脱氢酶基因（gdh）、吡咯喹啉醌依赖性葡萄糖脱氢酶基因（gcd）、柠檬酸合酶基因（gltA）与酒石酸脱氢酶基因（ttuC）等有机酸合成基因。研究为假单胞菌1616X1溶磷作用规律的阐释和基因改造等后期研究开发奠定基础。

关键词: 假单胞菌, 土壤溶磷, 有机酸, 基因组分析, 溶磷基因

Abstract: High efficiency phosphor-solublePseudomonassp. 1616X1 was used as the object and Ca3(PO4)2was used as the phosphorus source to explore the mechanism ofPseudomonasin dissolving insoluble inorganic phosphorus. The kinds and content of organic acids in the process of solubilizing phosphorus were determined by using high performance liquid chromatography (HPLC), the complete genome ofPseudomonassp. 1616X1 was sequenced by using the Illumina HiSeq X Ten sequencing platform, gene prediction, functional annotation, and analysis of phosphorus-solubilizing related genes were completed after sequence assembly. The results showed thatPseudomonassp. 1616X1 produced gluconic acid, tartaric acid, citric acid and other organic acids in the process of phosphorus dissolution, among which gluconic acid was the main one. The content of gluconic acid reached 17582.06 mg/L when the maximum amount of phosphorus was dissolved. The complete genome analysis revealed that the gene length ofPseudomonassp. 1616X1 was 5957 620 bp with 61.55% overall GC content, including 5535 coding sequences. Based on comparison and annotation by database of COG, GO and KEGG, the strain contained pyrroloquinoline quinone biosynthesis gene cluster (pqq), phosphate transport system synthesis genes (pstandpit) and phosphorus starvation response regulation genes (phoB,phoRandphoH). And it contained glucose dehydrogenase gene (gdh), pyrroloquinoline quinone-dependent glucose dehydrogenase gene (gcd), citrate synthase gene (gltA) and tartrate dehydrogenase gene (ttuC) and other organic acid synthesis genes. The findings of this study provided a foundation for understanding the mechanism of phosphate solubilization ofPseudomonassp. 1616X1 and future genetic modification research.

Key words: Pseudomonas, soil phosphorus solution, organic acids, genome analysis, phosphate solubilizing genes

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