生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 16-.doi: 10.3969/j.issn.2095-1736.2025.01.016

• 青藏高原植物资源研究与利用专题 • 上一篇    下一篇

以藏药红景天为碳源的碳点制备及表征研究

樊金昊1,2， 许仙明1,2， 孙桂鸿3， 郭明雄1,2   

  1. 1. 西藏大学 生态环境学院 青藏高原生物多样性与生态环境保护教育部重点实验室， 拉萨 850000；
    2. 武汉大学 生命科学学院 细胞稳态湖北省重点实验室， 武汉 430072；
    3. 武汉大学 泰康医学院(基础医学院) 过敏及免疫相关疾病湖北省重点实验室， 武汉 430071
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 郭明雄，博士，教授，研究方向为青藏高原特色藏药材和微生物资源调查及其保护利用，E-mail:guomx@whu.edu.cn
  • 作者简介:樊金昊，硕士研究生，研究方向为青藏高原特色藏药材和微生物资源调查及其保护利用，E-mail:1447387793@qq.com
  • 基金资助:
    西藏自治区中央引导地方科技发展资金项目（XZ202301YD0040C）； 国家自然科学基金地区科学基金项目（32260173）

Preparation and characterization of carbon dots using Tibetan medicine Rhodiola crenulate as carbon source #br#

FAN Jinhao1,2, XU Xianming1,2, SUN Guihong3, GUO Mingxiong1,2   

  1. 1. Key Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, School
    of Ecology and Environment, Tibet University, Lhasa 850000, China; 2. Hubei Key Laboratory of Cell Homeostasis,
    College of Life Sciences, Wuhan University, Wuhan 430072, China; 3. Hubei Provincial Key Laboratory of Allergy and
    Immunology, Taikang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan 430071, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 为探究不同溶剂提纯对红景天碳点性质的影响，通过水热法对藏药红景天粉末高温碳化，而后分离纯化制备红景天水溶性碳点（RWW-CDs）和红景天醇溶性碳点（RWE-CDs）。采用TEM、紫外-可见光光谱、荧光光谱、FTIR、XPS、XRD比较分析两种不同方法制备的红景天-CDs的表征数据。发现RWE-CDs的尺寸大于RWW-CDs。两种红景天碳点的荧光性能良好，但RWW-CDs的荧光性能优于RWE-CDs。XPS结果显示，两种红景天碳点的主要构成均为C、O、N等3种元素，RWE-CDs中N元素含量极低且C元素含量高于RWW-CDs。XRD结果显示，制备的两种红景天碳点结晶相与CQD的{10.0}平面一致，但RWE-CDs的特征峰优于RWW-CDs。分析其傅里叶红外光谱发现RWW-CDs的谱图出峰较为均一和稳定，RWE-CDs则杂峰较多，这可能是两种碳点提取的溶剂不同而造成的。同时使用CCK-8法和Calcein/PI染色法确定两种方法提纯的红景天碳点无明显生物学毒性差异。还对两种碳点的pH荧光稳定性和细胞成像进行了探究。

关键词: 藏药红景天, 碳点, TEM, XRD, XPS

Abstract: In an attempt to investigate the effect of different solvent purification on the properties ofRhodiola crenulatecarbon dots, in this study,Rhodiola crenulatewater-soluble carbon dots (RWW-CDs) andR.crenulatealcohol-soluble carbon dots (RWE-CDs) were prepared by hydrothermal carbonization ofR.crenulatepowder of the Tibetan medicine at high temperatures, which were then separated and purified. TEM, UV-visible spectroscopy, fluorescence spectroscopy, FTIR, XPS, and XRD comparative analyses were used to compare the characterization data ofR.crenulate-CDs prepared by the two different methods. The dimension of RWE-CDs was found to be larger than that of RWW-CDs, and the fluorescence properties of the twoR.crenulatecarbon dots were good, but the fluorescence properties of RWW-CDs were better than that of RWE-CDs. The result of XPS demonstrated that the main components of the twoR.crenulatecarbon dots were carbon, oxygen, and nitrogen, moreover, the content of nitrogen in the RWW-CDs was extremely low and the content of carbon was higher than that in the RWW-CDs. XRD results demonstrated that the twoR.crenulatecarbon dots prepared by this paper were characterized by a combination of the following factors. The crystalline phases of the twoR.crenulatecarbon dots prepared in this paper were consistent with the {10.0} plane of CQD, but the characteristic peaks of RWE-CDs were better than those of RWW-CDs. The Fourier infrared spectroscopy showed that the peaks of the RWW-CDs were more uniform and stable, while the RWE-CDs had more miscellaneous peaks, which might be due to the difference of solvents used for the two carbon dots extraction. The CCK-8 method and Calcein/PI staining method were also used to determine that there was no significant difference in biological toxicity between theR.crenulatecarbon dots purified by the two methods. Furthermore, the pH fluorescence stability and cellular imaging of the two carbon dots were also explored.

Key words: Tibetan medicineRhodiola crenulata, carbon dots, TEM, XRD, XPS

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