生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 9-.doi: 10.3969/j.issn.2095-1736.2025.01.009

• 青藏高原植物资源研究与利用专题 • 上一篇    下一篇

青稞干旱应答的转录组分析

陈帅豪1,2,3， 牛莉萍1,2,3， 博　拉1,2,3， 覃钟梦怡 1,2,3， 达瓦顿珠2， 伦珠朗杰2， 侯　昕1,2,3   

  1. 1. 西藏大学 生态环境学院， 拉萨 850000； 2. 省部共建青稞和牦牛种质资源与
    遗传改良国家重点实验室， 拉萨 850002； 3. 武汉大学 生命科学学院， 武汉 430072
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 侯昕，教授，研究方向为植物抗逆分子遗传学，E-mail：xinhou@whu.edu.cn
  • 作者简介:陈帅豪，硕士研究生，研究方向为青稞干旱适应性，E-mail：csh102050@126.com
  • 基金资助:
    省部共建青稞和牦牛种质资源与遗传改良国家重点实验室开放基金课题（XZNKY-CZ-2022-016-06）

Transcriptome analysis of the drought response in hulless barley

CHEN Shuaihao1,2,3, NIU Liping1,2,3, BO La1,2,3, QIN Zhongmengyi1,2,3, Dawa Dondup2,#br# Lhundrup Namgyal2, HOU Xin1,2,3   

  1. 1. School of Ecology and Environment, Tibet University, Lhasa 850000, China; 2. State Key Laboratory of Hulless
    Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, China; 3. School of Life Sciences,
    Wuhan University, Wuhan 430072, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 青稞是重要的禾本科作物，具有较强抗旱性，在青藏高原等地具有很高的经济和生态价值，为研究其抗旱机制，对两个抗旱性不同的青稞品种进行模拟干旱及转录组分析。从敏感品种YC85和抗旱品种ZY1100中分别筛选出85和186个差异表达基因。GO分析显示，YC85主要集中在水应答和离子转运，ZY1100富集在酸代谢和氨基酸合成；KEGG分析发现，YC85主要富集在MAPK信号和氨基酸代谢通路，ZY1100富集在激素信号转导和氨基酸合成。并从中挖掘到59个干旱应答基因。研究丰富了青稞抗旱基因信息，为解析青稞基因的表达调控机制及抗旱基因挖掘提供了宝贵资源，为基因功能研究和育种提供了重要依据。

关键词: 青稞, 干旱, 转录组分析, 差异表达基因, 胁迫应答

Abstract: Hulless barley is an important crop with high economic and ecological value on the Tibetan Plateau. It is highly resistant to drought stress. To study the molecular mechanism of drought resistance, two hulless barley varieties with different drought resistance were subjected to simulated drought and transcriptome analysis. After 24 h of drought treatment, 85 DEGs (differentially expressed genes) were screened from the drought-sensitive variety YC85, and 186 DEGs were screened from the drought-resistant variety ZY1100. GO enrichment analysis of the DEGs revealed that water response and ion transport were mainly enriched in YC85, and acid metabolism and amino acid biosynthesis were mainly enriched in ZY1100. KEGG enrichment analysis revealed that in YC85, MAPK signaling pathway-plant, alanine, aspartate and glutamate metabolism, etc., were enriched, and in ZY1100, phytohormone signaling, amino acid biosynthesis, etc., were the main enriched pathways. By analyzing the common and specific DEGs of the two hulless barley varieties, as well as the enrichment of GO functions and KEGG pathways, 59 genes involved in the drought response were identified. The results of this study provide valuable information and resources for understanding the regulatory mechanism of gene expression and the discovery of drought resistance genes in hulless barley, as well as providing an important basis for subsequent gene function research and breeding.

Key words: hulless barley, drought, transcriptome analysis, differentially expressed genes；stress response

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