生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 50-.doi: 10.3969/j.issn.2095-1736.2024.04.050

• 研究报告 • 上一篇    下一篇

宁北盐碱地土壤微生物的分离和抗菌活性筛选

马晓莉， 丁范范， 魏纪元， 周乐瑞， 胡运琪， 南泽东， 江志波   

  1. 北方民族大学 化学与化学工程学院 化工技术基础国家民委重点实验室， 银川 750021
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 江志波，博士研究生，副教授，主要研究方向为天然产物和微生物药学，E-mail:j836811@163.com
  • 作者简介:马晓莉，博士研究生，讲师，主要研究方向为天然产物和微生物药学，E-mail:2016030@nmu.edu.cn
  • 基金资助:
    宁夏自然科学基金项目(2023AAC03319)； 国家自然科学基金地区项目(82160672)； 北方民族大学科研项目(2022XYZHG03)； 北方民族大学研究生创新项目（YCX23171）

Extraction and antibacterial activity screening of soil microbes from saline-alkali soil of northern Ningxia

MA Xiaoli, DING Fanfan, WEI Jiyuan, ZHOU Lerui, HU Yunqi, NAN Zedong, JIANG Zhibo   

  1. Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission, School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 从宁北盐碱土壤中分离得到具有一定形态特征的单菌落45株，利用基因组提取技术，对菌株16S rRNA序列进行测序及菌属鉴定。结果显示，有16株放线菌，含13株链霉菌属，3株拟无枝酸菌属。抗菌活性实验结果表明，16株菌株均具有抑菌活性，其中，12株菌株次级代谢产物对普通变形杆菌具有显著抗菌活性，12株菌株次级代谢产物对金黄色葡萄球菌具有明显抑制活性，9株菌株次级代谢产物对白色念珠菌具有一定的生长抑制活性。研究表明，从宁北盐碱土壤中分离得到的微生物菌株具有较强抗菌或抑菌活性，不仅丰富了特殊生境微生物资源，也为进一步从活性菌株中分离抗菌活性先导化合物奠定了基础。

关键词: 盐碱地土壤, 微生物, 放线菌, 次级代谢产物, 抗菌活性

Abstract: 45 single colonies with certain morphological characteristics were isolated from saline-alkali soil in northern Ningxia, and the 16S rRNA sequence of the strains were sequenced and identified by genome extraction technology. Results showed that there were 16 strains of actinomycetes, including 13 strains of Streptomyces, 3 strains of Acidophilum. The experimental results of antibacterial activity exhibited that all 16 strains of actinomycetes had antibacterial activity, among which 12 strains of secondary metabolites had significant antibacterial activity againstProteus vulgaris, 12 strains of secondary metabolites had obvious inhibitory activity againstStaphylococcus aureus, and 9 strains of secondary metabolites had certain growth inhibitory activity againstCandida albicans. The researches demonstrated that the microbial strains isolated from saline-alkali soil in northern Ningxia had strong antibacterial or bacteriostatic activity. This study not only enriched the microbial resources in special habitats, but also laid a foundation for further isolation of antibacterial lead compounds.

Key words: saline-alkali soil, microorganisms, actinobacteria；secondary metabolites, antibacterial activity

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