生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (1): 73-.doi: 10.3969/j.issn.2095-1736.2024.01.073

• 研究报告 • 上一篇    下一篇

碳离子束辐照对马铃薯组培苗生长及生理特性的影响

王　琦1， 杨江伟1,2， 许超丽1,2， 周利斌3， 张　宁1,2， 司怀军1,2   

  1. 1. 甘肃农业大学 生命科学技术学院， 兰州 730070； 2. 甘肃农业大学 省部共建干旱生境作物学
    国家重点实验室， 兰州 730070； 3. 中国科学院近代物理研究所生物物理室， 兰州 730000
  • 出版日期:2024-02-18 发布日期:2024-02-18
  • 通讯作者: 司怀军，教授，从事马铃薯生物技术育种和分子生物学方面的研究，E-mail:hjsi@gsau.edu.cn
  • 作者简介:王琦，硕士研究生，从事马铃薯生物技术方面的研究，E-mail:1811519136@qq.com
  • 基金资助:
    甘肃省科技重大专项计划项目(GZGG-2021-6)； 科技部对发展中国家科技援助项目(KY201901015)； 中国科学院青年创新促进会项目(Y201974)

Effects of carbon ion beam irradiation on the growth, physiological characteristics of potato tissue culture plantlets

WANG Qi1, YANG Jiangwei1,2, XU Chaoli1,3, ZHOU Libin3, ZHANG Ning1,2, SI Huaijun1,2   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China;
    3. Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Online:2024-02-18 Published:2024-02-18

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摘要： 为研究高能碳离子束对马铃薯组培苗茎切段的影响，以马铃薯品种‘新大坪’的组培苗茎切段为研究材料，设置4个辐照剂量梯度对其进行辐照处理，测定植株致死率、生根率、侧芽形成率、形态变异率以及叶片生理指标，确定‘新大坪’组培苗茎切段的半致死辐照剂量。结果表明：辐照剂量与致死率之间的关系呈正相关性；组培苗茎切段的再生植株表型变化主要为叶片不对称、叶缘不规则、茎变异伸长、叶片丛生和叶片粘连等；随辐照剂量升高，超氧化物歧化酶、过氧化物酶和过氧化氢酶活性呈先升后降的趋势；丙二醛含量则呈先升后降，随后又升高的现象。利用线性回归方程计算‘新大坪’的半致死辐照剂量为20.37 Gy。

关键词: 高能碳离子束, 马铃薯组培苗, 辐照, 半致死辐照剂量, 生理特性

Abstract: To explore the effect of high-energy carbon ion beam on the stem section of potato tissue culture seedlings, this study took the stem section of potato variety “Xindaping” as the research material, four high-energy carbon ion beams with radiation dose gradient were selected. The plant lethal rate, rooting rate, lateral bud formation rate, morphological variation rate and leaf physiological and biochemical indicators were measured after irradiated. The semi-lethal radiation dose of the stem section of “Xindaping” tissue culture seedlings was determined. The results showed that there was a positive correlation between irradiation dose and lethal rate. The phenotypic changes of regenerated plants in stem segments of tissue culture seedlings were mainly as follows: asymmetric leaves, irregular leaf margins, variant elongation of stems, clustered leaves and adhesion of leaves. With the increase of irradiation dose, the activities of superoxide dismutase, peroxidase and catalase increased first and then decreased. The content of malondialdehyde increased first, then decreased, and last increased again. The semi-lethal irradiation dose of “Xindaping” was calculated by linear regression equation to be 20.37 Gy.

Key words: high-energy carbon ion beams, potato tissue cultured plantlets, irradiation, lethal dose at 50 percent, physiological characteristics

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