生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (1): 68-.doi: 10.3969/j.issn.2095-1736.2024.01.068

• 研究报告 • 上一篇    下一篇

椴叶鼠尾草抗烟草花叶病毒活性成分研究

王子夫1， 闫俊伊1， 刘　洋1， 吴江梅1， 闫晓慧1， 胡世俊2   

  1. 1. 西南林业大学 生物多样性保护学院 云南省森林灾害预警与控制重点实验室， 昆明 650224；
    2. 西南林业大学 林学院， 昆明 650224
  • 出版日期:2024-02-18 发布日期:2024-02-18
  • 通讯作者: 闫晓慧，博士，教授，研究方向为植物活性成分研究，E-mail:luckyyxh@163.com；胡世俊，博士，副教授，研究方向为入侵植物研究，E-mail:shijunhu@126.com；闫晓慧和胡世俊为共同通信作者
  • 作者简介:王子夫，硕士，研究方向为农业有害生物综合防控，E-mail:511810279@qq.com；闫俊伊，硕士，研究方向为植物检验检疫与生物安全，E-mail:754252940@qq.com；王子夫和闫俊伊为共同第一作者
  • 基金资助:
    国家自然科学基金项目（32160378）；云南省应用基础研究重点项目（2019FA011）；云南省高层次人才项目（YNWR-QNBJ-2018-300）

Antiviral components of Salvia tiliifolia against tobacco mosaic virus

WANG Zifu1, YAN Junyi1, LIU Yang1, WU Jiangmei1, YAN Xiaohui1, HU Shijun2   

  1. 1. Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Biodiversity Conservation,
    Southwest Forestry University, Kunming 650224, China; 2. College of Forestry, Southwest Forestry University,
    Kunming 650224, China
  • Online:2024-02-18 Published:2024-02-18

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摘要： 为寻找新型植物病毒抑制剂，以烟草花叶病毒（TMV）为供试病毒，研究发现入侵植物椴叶鼠尾草提取物具有抗TMV活性。通过半叶枯斑法不同施药方式筛选发现，在质量浓度为1 mg/mL时，石油醚萃取物的保护作用和钝化作用最强，抑制率为40.95%和52.85%；乙酸乙酯萃取物的治疗作用最强，抑制率为52.85%，与阳性对照药剂宁南霉素无显著差异。石油醚萃取物各馏分中，Fr7的保护作用和治疗作用最好，抑制率为40.51%和45.52%，Fr6的钝化作用最好，抑制率为74.63%。乙酸乙酯萃取物各馏分中，Fr1′的保护作用最强，抑制率为61.77%，Fr3′的治疗作用最强，抑制率为55.02%，Fr4′的钝化作用最强，抑制率为60.22%。运用现代色谱波谱技术分离鉴定了5个化合物分别为tilifodiolide、eupatoric acid、熊果酸、齐墩果酸和2-甲基-5,7-二羟基色原酮。

关键词: 椴叶鼠尾草, 化学成分, 分离纯化, 烟草花叶病毒（TMV）, 抗TMV活性

Abstract: In order to find new plant virus inhibitors, tobacco mosaic virus (TMV) was used as the test virus. It was found that the extract of Salvia tiliifolia, an invasive plant, showed anti-TMV activity. Screening by the half-leaf method along with different treatment pattern of extract, the petroleum ether extract showed the strongest protective effect and inactivation effect at the concentration of 1 mg/mL, with inhibition rate of 40.95% and 52.85% respectively. The ethyl acetate extract showed the best curative effect with the inhibition rate of 52.85%, which was not significantly different from Ningnanmycin, a positive control. Among fractions of petroleum ether extracts, Fr7 showed the best protective effect and curative effect, with the inhibition rate of 40.51% and 45.52% respectively, and Fr6 showed potent inactive effect with the inhibition rate of 74.63%. Among fractions of ethyl acetate extracts, Fr1′ showed the strongest protective effect with an inhibition rate of 61.77%, Fr3′ displayed the best curative effect with an inhibition rate of 55.02%, and Fr4′ showed potent inactive effect with the inhibition rate of 60.22%. Five compounds were separated and identified by modern chromatographic spectrum technologies, they were tilifodiolide, eupatoric acid, ursolic acid, oleanolic acid, and 2-methyl-5,7-dioxy chromone.

Key words: Salvia tiliifolia, chemical constituents, separation and purification, tobacco mosaic virus(TMV), anti-TMV activity

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