生物学杂志

生物学杂志

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在淡水和海水环境下虹鳟血液理化指标、肌肉和肝脏的抗氧化酶活性的比较

  

  1. 宁波大学 应用海洋生物技术教育部重点实验室， 宁波 315211
  • 出版日期:2016-08-18 发布日期:2016-08-18
  • 通讯作者: 徐善良，教授，硕士生导师，从事海洋生物研究,E-mail: xushanliang@nbu.edu.cn
  • 作者简介:巩建华，硕士研究生，从事鱼类繁殖生物学,E-mail: 17855847980@163.com
  • 基金资助:
    国家海洋局2013年海洋经济创新区域示范项目（F01521145300）

Comparison of blood parameters, antioxidant enzyme activity in liver and muscle of rainbow trout (Oncorhynchus mykiss) following exposure in freshwater and seawater

  1. Key Laboratory of Applied Marine Bitechnology Ministry of Education, Ningbo University, Ningbo 315211, China
  • Online:2016-08-18 Published:2016-08-18

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摘要： 采用贝克曼电解质分析仪和日立H-7600全自动生化分析仪，对不同环境下虹鳟血液理化指标、肌肉和肝脏抗氧化酶活性进行比较。结果表明： 1） 海、淡水环境下，虹鳟血清中谷丙转氨酶（GPT）含量差异显著（P<0.05），谷草转氨酶(GOT)含量差异也显著（P<0.05），淡水组均大于海水组，其值是分别是（7.00±2.82）μ/L、（44.00±7.43）μ/L，分别是海水组的1.36倍和3.35倍。2） 微量元素钾（K+）、钠（Na+）、氯（Cl-）、钙（Ca2+）和白蛋白（A）、甘油三酯以及球蛋白（G）含量，海水组大于淡水组；总胆固醇含量差异显著（P<0.05），海水组大于淡水组。3）海水组血红蛋白含量、血小板压积、红细胞数目、血小板数目均大于淡水组，其值分别降低了68.31%、37.32%、22.58%、24.29%；血小板分布宽度、红细胞分布宽度、红细胞压积差异显著（P<0.05）。

关键词: 虹鳟鱼, 血液理化指标, 抗氧化酶

Abstract: Using Beckman electrolyte and automatic biochemical analyzer, we compared the blood index, and the activity of antioxidant enzyme in muscle and liver under different circumstance . It turned out that under sea and fresh water environment, the content of glutamic pyruvic transaminase（GPT）are significantly different（P<0.05）, so are the contents of aspartate transaminase（GOT）. The contents of fish in GDT and GOT in freshwater are higher than those of seawater, the values are （7.00±2.82）μ/ L and（44.00±7.43）μ/ L, respectively, which are 1.36 times and 3.35 times of the seawater group. The trace element potassium （K+）, sodium （Na+）, chlorine（Cl-）, calcium （Ca2+) and albumin（A）, triglycerides and globulin（G）in sea wafer fish are greater than those in freshwater group. Total cholesterol levels were markedly different（P <0.05）, the level of seawater group is greater than that of freshwater group. Hemoglobin, platelet hematocrit, number of red blood cells, platelet count of fishes in seawater are obviously higher than those in freshwater group, which decreased by 68.31%, 37.32%, 22.58% and 24.29%, respectively; The width of platelet distribution and red blood cell distribution and the hematocrit is apparently different（P<0.05）.

Key words: rainbow trout, the blood index, antioxidant enzymes