生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (1): 37-.doi: 10. 3969/ j. issn. 2095-1736. 2022. 01. 037

• 研究报告 • 上一篇    下一篇

贝莱斯芽孢杆菌对花生白绢病的防治效果

  

  1. 河南省科学院生物研究所, 郑州 450008
  • 出版日期:2022-02-18 发布日期:2022-02-15
  • 通讯作者: 张英涛,高级工程师,主要从事植物病害生防菌研究,E-mail:2251316831@qq.com
  • 作者简介:潘梦诗,硕士,研究实习员,研究方向为生物防治,E-mail:961929691@ qq. com
  • 基金资助:
    河南省科学院科研开发专项基础科研业务费(200605066);河南省科学院省级特聘研究员项目(200505006)

Biocontrol effects of Bacillus velezensis on peanut stem rot caused by Sclerotium rolfsii

  1. Henan Academy of Science Institute of Biology, Zhengzhou 450008, China
  • Online:2022-02-18 Published:2022-02-15

PDF (PC)

33

摘要：

贝莱斯芽孢杆菌作为最新发现的潜力生防菌,通过平板对峙试验、生防机制初探和盆栽试验,研究其对花生白绢病的防治效果。平板对峙试验结果表明,贝莱斯芽孢杆菌可显著抑制花生白绢病的生长,菌丝生长抑制率达81. 93%,破坏病菌菌丝结构,抑制菌核的生成。对贝莱斯芽孢杆菌的生防机制进行初探,贝莱斯芽孢杆菌可产生蛋白酶、纤维素酶、淀粉酶和嗜铁素,其无菌发酵滤液具有显著的拮抗作用。盆栽试验结果显示,经菌液处理后均能抑制花生白绢病的发生,降低花生病情指数,对花生的生长有一定的促进作用,以接种病菌2 d 后喷洒菌液的处理效果最优,防治效果可达66. 15%。贝莱斯芽孢杆菌可用于花生白绢病的防治,具有良好的生防前景


关键词: 贝莱斯芽孢杆菌, 花生白绢病, 拮抗, 促生, 生防

Abstract:

As the potential biocontrol bacteria, Bacillus velezensis was studied for its biological control of peanut stem rot caused by Sclerotium rolfsii with coculture method, research of antagonistic mechanism and pot experiments. By coculture method on PDA, B.velezensis can inhibit the hyphal growth of S. rolfsii significantly, with an inhibition rate of 81. 93%, destroy the structure of the pathogenic mycelium and prevent the formation of sclerotia. In order to understand antagonistic mechanism of B. velezensis, it was found that B. velezensis can produce protease, cellulase, amylase and siderophore, which aseptic fermentation filtrate also has an antagonistic effect. Under pot experiments the treatments with bacteria liquid can inhibit the occurrence of peanut stem rot caused by S. rolfsii, reduce the peanut disease index, and have a certain promotion effect on the growth of peanuts. Peanuts which colonizated with S. rolfsii for 2 days and then sprayed by the bacteria liquid exhibited greater than other treatments with 66. 15% biocontrol efficacy against S.rolfsii. B. velezensis can be used for the prevention and treatment of peanut stem rot caused by S. rolfsii, and has a good prospect for biocontrol.

Key words: Bacillus velezensis, Sclerotium rolfsii, antagonistic, growth promoting, biological control

中图分类号: