生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (5): 11-.doi: 10.3969/j.issn.2095-1736.2026.05.011

• 研究报告 • 上一篇    下一篇

高盐高氨氮双胁迫下盐单胞菌MY-11的筛选及脱氮特性

张宇红， 刘　健， 蔡佳诚， 张　放， 姜月晴   

  1. 沈阳化工大学 环境与安全工程学院， 沈阳 110142
  • 出版日期:2026-10-18 发布日期:2026-10-20
  • 作者简介:张宇红，博士，副教授，研究方向为水污染控制与治理、环境生物技术，E-mail：zhangyuhong@syuct.edu.cn
  • 基金资助:
    辽宁省应用基础研究计划项目（2023JH2/101300047）

Screening and denitrification characteristics of Halomonas sp. MY-11 under dual stress of high salinity and high ammonia nitrogen

ZHANG Yuhong, LIU Jian, CAI Jiacheng, ZHANG Fang, JIANG Yueqing   

  1. College of Environmental and Safety Engineering, Shenyang University of Chemical Technology,
    Shenyang 110142, China
  • Online:2026-10-18 Published:2026-10-20

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摘要： 研究从化工废水中筛选出一株在高盐高氨氮双胁迫下高效脱氮的异养硝化—好氧反硝化（HN-AD）菌株Halomonassp. MY11。通过单因素实验系统考察菌株MY-11的生长特性与脱氮性能，菌株在所设置的不同环境条件下均能生长并具有HN-AD能力。确定其最适培养条件为：碳源为丁二酸钠、C/N 10~18、pH 7~9、摇床转速140~220 r/min、培养温度30~34 ℃、接种量1%~5%（体积比）、盐度为2%~8%。在此条件下，菌株对NH+4-N和NO－3-N的去除率在30 h内均可达99%以上。菌株能够在盐度4%、氨氮质量浓度1 200 mg/L的高盐高氨氮条件下生长并具有较高的脱氮效率，也能够适应多种无机盐环境。研究表明，菌株MY-11具有优异的HN-AD性能，在高盐高氮工业废水生物处理领域展现出显著的应用价值。

关键词: 异养硝化—好氧反硝化, 耐盐, 耐高氨氮, 生物脱氮, 复杂废水

Abstract: A heterotrophic nitrification-aerobic denitrification （HN-AD） strain,Halomonassp. MY11, with tolerance to high salt and high ammonia nitrogen was successfully screened from a chemical wastewater treatment plant. The growth characteristics and denitrification performance of strain MY-11 were investigated by a one-factor-at-a-time experimental design, and the strain was able to grow and exhibit HN-AD activity under different environmental conditions, and the optimal culture conditions were determined as follows: carbon source of sodium succinate, C/N of 10－18, pH 7－9, shaker speed of 140－220 r/min, incubation temperature of 30－34 ℃, inoculum amount of 1%－5% （volume ratio）, and salinity of 2%－8%. Under these conditions, the removal efficiency of the strain for both NH+4-N and NO－3-N reached more than 99% within 30 h. The strain could grow under high-salt （4% salinity） and high-ammonia-nitrogen （1200 mg/L） conditions, exhibited high nitrogen removal efficiency, and could also adapt to various inorganic salt environments. It is demonstrated that strain MY-11 had excellent HN-AD performance and showed significant application value in the field of biological treatment of high-salt and high-nitrogen industrial wastewater.

Key words: heterotrophic nitrification-aerobic denitrification, salt tolerance, high ammonia nitrogen tolerance, biological nitrogen removal, complex wastewater

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