生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (1): 61-.doi: 10.3969/j.issn.2095-1736.2025.01.061

• 研究报告 • 上一篇    下一篇

沙蓬粗提物对陇南花椒黑胫病病原菌抑制作用研究

史　梁1,2,3， 钱朝菊1,2， 范兴科1,2， 燕　霞4， 曹永红5， 张　洪6， 陆　欣6， 马小飞1,2   

  1. 1. 中国科学院西北生态环境资源研究院 干旱区生态安全与可持续发展重点实验室， 兰州 730000;
    2. 中国科学院西北生态环境资源研究院 甘肃省寒区旱区逆境生理与生态重点实验室， 兰州 730000;
    3. 中国科学院大学， 北京 100049; 4. 中国科学院西北生态环境资源研究院 中国科学院内陆河流域
    生态水文重点实验室， 兰州 730000; 5. 陇南市经济林研究院， 陇南 742100;
    6. 甘肃育霖陇低碳节能科技有限公司， 兰州 730000
  • 出版日期:2025-02-18 发布日期:2025-02-12
  • 通讯作者: 马小飞，博士，教授，研究方向为干旱区生态农业，E-mail:maxiaofei@lzb.ac.cn
  • 作者简介:史梁，硕士研究生，研究方向为沙生资源植物的开发与应用，E-mail:shiliang@nieer.ac.cn
  • 基金资助:
    中国科学院战略生物资源计划项目(KFJ-BRP-007-015)； 国家自然科学基金项目(32271695)； 甘肃省省级引导科技创新发展专项(Y939BD1001)； 兰州市人才创新创业项目(2021-RC-76)； 兰州市青年科技人才创新项目(2023-QN-140)

Study on the inhibitory effect of crude extract of Agriophyllum squarrosum on the pathogen of blackshank disease of Zanthoxylum bungeanum in Longnan

SHI Liang1,2,3, QIAN Chaoju1,2, FAN Xingke1,2, YAN Xia4, CAO Yonghong5,ZHANG Hong6, LU Xin6, MA Xiaofei1,2   

  1. 1. Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Northwest Institute of
    Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 2. Key Laboratory of
    Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Northwest Institute of Eco-Environment
    and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 3. University of Chinese Academy of Sciences,
    Beijing 100049, China; 4. Key Laboratory of Inland River Ecohydrology, Cold and Arid Regions Environmental and
    Engineering Research, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences,
    Lanzhou 730000, China; 5. Longnan Academy of Non-wood Forest, Longnan 742100, China; 6. Gansu Yulinlong
    Low Carbon Energy Saving Technology Co., Ltd., Lanzhou 730000, China
  • Online:2025-02-18 Published:2025-02-12

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摘要： 为了明确病原菌，采用形态学和ITS测序的方法对陇南地区花椒黑胫病病原菌进行分离和鉴定，从多株陇南花椒的黑胫病病灶部位分离纯化得到13种不同菌落形态特征的菌株，经过寄主回接，科赫法则验证，首次明确陇南地区花椒黑胫病病原菌为一种极细链格孢菌。通过梯度生长抑制测定法评估沙蓬粗提物对该极细链格孢菌的抑制效果，发现8 mg/mL的沙蓬粗提物对极细链格孢菌的抑制效果最佳，抑制率达到70.18%。研究结果不仅说明花椒黑胫病产生的机制复杂多样，同时说明沙生药用植物沙蓬的粗提物作为植物源杀菌剂在重要经济林作物主要病害的生物防治中具有广阔前景，拓展了沙蓬在我国特色生态农业中的应用方向。

关键词: 沙蓬粗提物, 花椒黑胫病, 病原菌, 极细链格孢菌, 植物源杀菌剂

Abstract: In order to clarify the pathogenic fungi, morphological and ITS sequencing methods were used to isolate and identify the pathogenic fungi of black shank disease onZanthoxylum bungeanumin Longnan area. 13 strains with different colony morphological characteristics were isolated and purified from the black shank disease lesions of multiple LongnanZ. bungeanumstrains. After host tieback and Koch’s rule verification, it was clarified for the first time that the causative agent ofZ. bungeanumblack shank in Longnan was a type ofAlternaria tenuissima. The inhibitory effect of the crude extract ofAgriophyllum squarrosumagainstA. tenuissimawas evaluated by gradient growth inhibition assay. It was found that the crude extract of 8 mg/mL ofA. squarrosumhad the best inhibitory effect onA. tenuissimaand the inhibition rate reached at 70.18%. The research case not only illustrated the complex and diverse mechanisms of the occurrence of black shank inZ. bungeanum, but also supported that the crude extract of the psammophila medicinal plantA. squarrosumhad broad prospects as a botanical fungicide in the biological control of major diseases of important economic forest crops, and it expanded the application direction ofA. squarrosumin China’s characteristic ecological agriculture.

Key words: crude extract ofAgriophyllum squarrosum, black shank disease ofZanthoxylum bungeanum, pathogen;Alternaria tenuissima, fungicides of plant origin

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