生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (5): 89-.doi: 10.3969/j.issn.2095-1736.2024.05.089

• 研究报告 • 上一篇    下一篇

褪黑素对猪胎盘滋养层细胞增殖及功能的影响

王佳琪1,2,3， 刘　彦2,3， 郑　琛1， 冯　涛1,2,3   

  1. 1. 甘肃农业大学 动物科学技术学院， 兰州 730070； 2. 北京市农林科学院畜牧兽医研究所，
    北京 100097； 3. 北京市农林科学院-美国俄克拉荷马州立大学动物科学联合实验室， 北京 100097
  • 出版日期:2024-10-18 发布日期:2024-10-14
  • 通讯作者: 冯涛，博士，研究员，研究方向为动物繁殖调控，E-mail:fengtao_gs@163.com
  • 作者简介:王佳琪，硕士研究生，研究方向为繁殖营养调控，E-mail:2601396139@qq.com
  • 基金资助:
    国家自然科学基金项目（31972575）； 北京市农林科学院课题（CZZJ202205）

Effect of melatonin on proliferation and function of porcine placental trophoblast cells

WANG Jiaqi1,2,3, LIU Yan2,3, ZHENG Chen1, FENG Tao1,2,3   

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2. Institute
    of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences
    (BAAFS), Beijing 100097, China; 3. Joint Laboratory of Animal Science between IAHVM of BAAFS and Division of
    Agricultural Science and Natural Resource of Oklahoma State University, Beijing 100097, China
  • Online:2024-10-18 Published:2024-10-14

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摘要： 旨在研究褪黑素对猪胎盘滋养层细胞（porcine placental trophoblast cells，pTr）增殖和功能的影响。不同浓度褪黑素（0、10 nmol/L、100 nmol/L、1 μmol/L、10 μmol/L、100 μmol/L）处理pTr细胞48 h后，通过测定细胞数、VEGF、EGF、E2、P4分泌水平及相关基因mRNA表达水平，分析褪黑素对pTr细胞增殖及功能的影响。结果显示，处理48 h时，10 μmol/L的褪黑素显著促进pTr增殖（P<0.05），并显著提高VEGF、EGF、E2和P4分泌水平（P<0.05）。与空白对照组相比，10 μmol/L褪黑素显著上调MT1、CDK4、SLC2A1、VEGF及EGFR的表达（P<0.05），并极显著上调VEGFR2、EGF、ESR1和PGR的表达（P<0.01）。综上所述，10 μmol/L的褪黑素能显著促进pTr增殖，其可能通过与MT1结合直接，或通过上调CDK4、SLC2A1基因表达间接促进pTr细胞的增殖；此外，褪黑素还可能通过上调pTr中生长因子及其受体、类固醇激素受体等基因表达，以及促进生长因子、生殖激素合成和分泌改善猪胎盘功能。

关键词: 褪黑素, 猪胎盘滋养层细胞, 细胞增殖, 基因表达

Abstract: To investigate the effects of melatonin on the proliferation and function of porcine placenta trophoblast cells (pTr), pTr cells were treated with different concentrations of melatonin (0, 10 nmol /L, 100 nmol/L, 1 μmol/L, 10 μmol/L and 100 μmol/L) for 48 hours. The effect of melatonin on the proliferation and function of pTr cells was analyzed by detecting the number of cells, the secretion levels of VEGF, EGF, E2, P4 and the mRNA expression levels of related genes. The results showed that the concentration of 10 μmol/L melatonin significantly promoted the proliferation of pTr (P<0.05). The secretion levels of VEGF, EGF, E2 and P4 were significantly increased (P<0.05). Compared with the blank control group, 10 μmol/L melatonin significantly up-regulated the expression ofMT1,CDK4,SLC2A1,VEGF, andEGFRin pTr cells (P<0.05), and the expression ofVEGFR2,EGF,ESR1, andPGR(P<0.01). In conclusion, 10 μmol/L melatonin could promote the proliferation of pTr cells by directly binding to MT1, or indirectly by regulating the expression ofCDK4andSLC2A1genes. In addition, melatonin might also improve placental function in pigs by regulating the expression of growth factors and their receptors and steroid hormone receptors and promoting the synthesis and secretion of growth factors and reproductive hormones.

Key words: melatonin, porcine placental trophoblast cells, cell proliferation, gene expression

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