生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (5): 84-.doi: 10.3969/j.issn.2095-1736.2024.05.084

• 研究报告 • 上一篇    下一篇

马来穿山甲个体识别STR分子标记的开发及应用

黄娅琳1， 梁　成2， 徐燕红1， 陈云霞1， 黄　捷3   

  1. 1. 南京警察学院 刑事科学技术学院， 南京 210023； 2. 云南省公安厅 生态环境与食品药品犯罪侦查
    总队， 昆明 650224； 3. 广西壮族自治区公安厅森林警察总队， 南宁 530028
  • 出版日期:2024-10-18 发布日期:2024-10-14
  • 作者简介:黄娅琳，博士，教授，主要从事野生动物DNA鉴定及相关遗传学研究，E-mail:huangyalin228@163.com
  • 基金资助:
    中央高校基本科研业务费专项资金项目(LGZD202105)； 2021江苏高校“青蓝工程”

Development and application of STR molecular markers of pangolin for individual identification

HUANG Yalin1, LIANG Cheng2, XU Yanhong1, CHEN Yunxia1, HUANG Jie3   

  1. 1. College of Criminal Science and Technology, Nanjing Police College, Nanjing 210023, China; 2. Yunnan Public
    Security Department Ecological Environment, Food and Drug Crime Investigation Corps, Kunming 650224, China;
    3. Forest Police Corps of Guangxi Zhuang Autonomous Region Public Security Department, Nanning 530028, China
  • Online:2024-10-18 Published:2024-10-14

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摘要： 通过对76只马来穿山甲个体的DNA进行STR位点的多态性分析和STR片段检测，筛选出16个可用于马来穿山甲特异性个体识别的STR分子标记；开发出相应的STR引物序列，并结合荧光PCR复合扩增技术和毛细管电泳技术，构建了一套针对马来穿山甲的快速个体识别方法。盲测实验验证结果表明，所筛选出的16个STR位点具有很高的鉴别准确性，可用于马来穿山甲的个体识别。盲测实验所取样本有肌肉、内脏、鳞甲，所筛选的STR分子标记扩增稳定性较好，适用于各类涉案组织检材的检验鉴定。该方法扩增片段较短，尤其适用于DNA含量较低或高度降解的马来穿山甲鳞甲检材。该方法将解决偷猎、运输、贩卖、食用、药用马来穿山甲案件的准确量刑问题，也将为马来穿山甲的保护遗传学研究奠定基础。

关键词: 马来穿山甲, STR, 分子标记, 个体识别, 量刑

Abstract: STR polymorphism analysis was performed on Malay pangolins based on 76 individuals. 16 STR molecular markers were selected for specific individual identification of Malay pangolins. To develop corresponding STR primer sequences, a rapid individual identification method for Malay pangolins was constructed by combining fluorescence PCR composite amplification technology and capillary electrophoresis technology. Through blind testing experiments, the results showed that the 16 selected STR loci had high identification accuracy and could be used for individual identification of Malay pangolins. The samples taken in the blind test experiment included muscles, viscera, and scales. The results showed that the selected STR molecular markers had good amplification stability and were suitable for the inspection and identification of various types of tissue samples. Due to the short amplification fragments, this method is particularly suitable for Malay pangolin scale nail samples with low or highly degraded DNA. It will solve the problems of accurate sentencing in cases of poaching, transportation, trafficking, edible and medicinal Malay pangolins, and will also contribute to the conservation biology research of Malay pangolins.

Key words: Malay pangolin, STR, molecular marker, individual identification, measurement of penalty

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