生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 83-.doi: 10.3969/j.issn.2095-1736.2024.04.083

• 研究报告 • 上一篇    下一篇

光照对仙人掌科植物蓬莱宫中磷酸钙晶体形貌的影响

李昌颖1， 秦利鸿2， 郑登月2， 杜　倩1， 陈春丽2， 文晓鹏1   

  1. 1. 贵州大学 生命科学学院 农业生物工程研究院 山地植物资源保护与保护种质创新教育部重点实验室 
    山地生态与农业生物工程协同创新中心， 贵阳 550025； 2. 华中农业大学 生命科学技术学院， 武汉 430070
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 文晓鹏，博士，教授，研究方向为果树生物技术与遗传育种，E-mail:xpwensc@hotmail.com；陈春丽，博士，教授，研究方向为植物分子细胞发育生物学与细胞遗传学，E-mail:chenchunli@mail.hzau.edu.cn；文晓鹏和陈春丽为共同通信作者
  • 作者简介:李昌颖，硕士研究生，研究方向为植物生物矿化，E-mail:1280049785@qq.com
  • 基金资助:
    国家自然科学基金项目（32060663）

Effect of light on the morphological features of calcium phosphate crystals in the Cactaceae plant Mammillaria schumannii #br#

LI Changying1, QIN Lihong2, ZHENG Dengyue2, DU Qian1, CHEN Chunli2, WEN Xiaopeng1   

  1. 1. Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry
    of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB),
    College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;
    2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 研究发现仙人掌科植物——蓬莱宫中存在花束状的晶体，在此基础上对蓬莱宫疣部进行扫描电镜观察和能谱分析，结果表明，该矿物晶体含O、Ca、P元素，该类矿物质为磷酸钙晶体，主要镶嵌于皮层细胞壁上，极少分布在细胞中，其形貌大多呈四周散开或向一个方向展开的花束状，偶然观察到有膜包裹的橄榄球状；对蓬莱宫弱光处理后观察发现，表型明显发生变化，颜色从深绿变为嫩绿，茎伸长，疣间距变宽，变细长，刺较软向四周微张；疣中晶体密度显著减少，且磷酸钙晶片小部分聚集在一起，晶体尺寸也显著缩小（P<0.001）。研究结果为对植物中磷酸钙的形貌特征及其功能的理解提供了参考。

关键词: 生物矿化, 蓬莱宫, 磷酸钙, 形貌特征, 扫描电镜

Abstract: It was found that there were calcium phosphate crystals inMammillaria schumannii. The tubercles ofM. schumanniiwere observed using a scanning electron microscope and an X-ray energy spectrum. The results showed that the mineral crystals, contained the O, Ca, and P elements, which were calcium phosphate and mainly embedded in the cortical cell wall with sporadic distribution in the cells. The majority of their morphological features resemble bouquets dispersed in all directions or just one, and rarely, olive balls coated with membranes could be seen. After weak-light treatment inM. schumannii, it was found that the crystal density in tubercles decreased obviously, a little amount of calcium phosphate crystals gathered together, and the overall size also decreased significantly (P<0.001). The results here provided a better understanding of the morphological characteristics of plant calcium phosphate as well as their roles in plants.

Key words: biomineralization;Mammillaria schumannii, calcium phosphate, morphological feature microscopy, scanning electron microscopy

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