生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 88-.doi: 10.3969/j.issn.2095-1736.2024.04.088

• 研究报告 • 上一篇    下一篇

转录因子NF-κB对鲤疱疹病毒Ⅱ型基因启动子的表达调控研究

谢雅晴1， 龙　晨1， 吕利群2   

  1. 1. 上海海洋大学 国家水生动物病原库， 上海 201306；
    2. 上海海洋大学 农业农村部淡水水产种质资源与利用重点实验室， 上海 201306
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 吕利群，博士，教授，主要从事水产动物病原学研究，E-mail:lqlv@shou.edu.cn
  • 作者简介:谢雅晴，硕士研究生，研究方向为分子病毒学，E-mail:xieyq901277@163.com
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-45-19)

Regulation of the gene promoter of Cyprinid herpesvirus 2by the transcription factor NF-κB

XIE Yaqing1, LONG Chen1, LYU Liqun2   

  1. 1. National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China;
    2. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs,
    Shanghai Ocean University, Shanghai 201306, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 为深入探究NF-κB信号通路对CyHV-2感染过程的影响，在CyHV-2感染的GiCF细胞中加入NF-κB激动剂，通过RT-qPCR和Western Blot分析在感染24、72、96 h后CyHV-2编码基因在转录和翻译水平的表达情况。结果显示，在加入NF-κB激动剂后的72、96 h，CyHV-2编码基因转录和翻译水平均明显高于对照组，提示NF-κB促进CyHV-2编码基因的转录及翻译。为进一步阐明NF-κB如何影响CyHV-2的转录和翻译，利用生物信息学软件预测CyHV-2部分编码基因的启动子序列中和NF-κB的结合位点，采用双荧光素酶报告基因实验分析NF-κB对启动子活性的影响。结果表明，NF-κB过表达提高了病毒基因启动子活性，提示NF-κB可以通过促进CyHV-2基因的启动子活性来增强病毒复制水平。研究结果有助深入探究CyHV-2基因的表达翻译机制，也为开发基于NF-κB信号通路靶点的新的抗病毒抑制剂提供理论支撑。

关键词: 鲤疱疹病毒Ⅱ型, NF-κB, 启动子, 转录调控, 双荧光素酶报告基因

Abstract: In order to investigate the mechanism of NF-κB in enhancing CyHV-2 replication, transcriptional and translational levels of CyHV-2 genes at 24, 72 and 96 h post CyHV-2 infection in the presence of NF-κB agonist were monitored by RT- qPCR and Western Blot assays.The results showed that the transcriptional levels and protein levels of CyHV-2 genes were increased at 72 and 96 h in the presence of NF-κB agonist.To further elucidate how NF-κB affects the transcription and translation of CyHV-2, the binding sites of the promoter region for NF-κB were predicted and dual luciferase reporter gene experiments were performed to study the regulatory role of NF-κB on viral gene expression.The experimental results showed that NF-κB overexpression increased CyHV-2 promoter activity, further suggesting that NF-κB promotes viral replication by activating the promoter of CyHV-2 genes.This study should provide novel strategies for anti-CyHV-2 drugs and enhance the understanding of CyHV-2 replication.

Key words: CyHV-2, NF-κB, promoter, transcriptional regulation, dual luciferase reporter gene

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