生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (3): 34-.doi: 10.3969/j.issn.2095-1736.2024.03.034

• 生物冶金专题 • 上一篇    下一篇

中度嗜热菌Leptospirillum ferrooxidans和Acidithiobacillus caldus对砷的耐受性研究

陈彦臻1,2， 张广积1,2， 郭嘉乐1,3， 杨　超1,2   

  1. 1. 中国科学院过程工程研究所 中国科学院绿色过程与工程重点实验室, 北京 100190；
    2. 中国科学院大学 化学工程学院, 北京 100049； 3. 合肥大学 生物食品与环境学院, 合肥 230601
  • 出版日期:2024-06-18 发布日期:2024-06-17
  • 通讯作者: 张广积，博士，研究员，研究方向为生物冶金，E-mail:gjzhang@ipe.ac.cn
  • 作者简介:陈彦臻，硕士研究生，研究方向为生物冶金，E-mail:chenyanzhen21@ipe.ac.cn
  • 基金资助:
    国家重点研发计划项目（2022YFC2105302）

Study on arsenic tolerance of moderately thermophilic bacteria Leptospirillum ferrooxidans and Acidithiobacillus caldus

CHEN Yanzhen1,2, ZHANG Guangji1,2, GUO Jiale1,3, YANG Chao1,2   

  1. 1. CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy
    of Sciences, Beijing 100190, China; 2. School of Chemical Engineering, University of Chinese Academy of Sciences,
    Beijing 100049, China; 3. School of Biology, Food and Environment, Hefei University, Hefei 230601, China
  • Online:2024-06-18 Published:2024-06-17

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摘要： 以难处理金矿生物预氧化过程中使用的L. ferrooxidans（Leptospirillum ferrooxidans, 氧化亚铁钩端螺旋菌）和A. caldus（Acidithiobacillus caldus, 喜温嗜酸硫杆菌）这2种中度嗜热菌为主要研究对象，通过摇瓶实验研究不同价态的砷\[As（Ⅲ）和As（Ⅴ）\]对2种中度嗜热菌生物氧化处理金矿效果的影响，并与A. ferrooxidans（Acidithiobacillus ferrooxidans, 嗜酸氧化亚铁硫杆菌）和T. thiooxidans（Thiobacillus thiooxidans, 氧化硫硫杆菌）这2种嗜中温菌进行比较。结果表明，中度嗜热菌对As（Ⅲ）的耐受性明显弱于嗜中温菌，但对As（Ⅴ）的耐受性与嗜中温菌接近。同时，As（Ⅲ）对铁氧化细菌生长的影响大于硫氧化细菌。实验发现，提高菌种接种量并对细菌进行驯化有利于中度嗜热菌对砷毒性的适应，在实际矿浆体系中通过提高接种量可以使中度嗜热菌最终适应高砷环境，最高质量浓度可达约1.54 g/L，获得较好的矿物氧化浸出效果，砷浸出率达到70%以上仅需10 d。

关键词: 生物氧化, 中度嗜热菌, 砷价态, 驯化, 菌种接种量

Abstract: In this study, two moderately thermophilic bacteria, includingL. ferrooxidans(Leptospirillum ferrooxidans) andA. caldus(Acidithiobacillus caldus), used in bioleaching processes of refractory gold ores were taken as the main research objects. The effects of valence of arsenic (As(Ⅲ) and As(Ⅴ)) on the bio-oxidation of two moderately thermophilic bacteria were investigated through studied by shake flask experiment. The results were compared with two mesophilic bacteria,A. ferrooxidans(Acidithiobacillus ferrooxidans) andT. thiooxidans(Thiobacillus thiooxidans). The results showed that the tolerance to As(Ⅲ) of moderately thermophilic bacteria was significantly weaker than that of mesophilic bacteria, but both of thermophilic and mesophilic bacteria showed similar tolerance to As(Ⅴ). At the same time, the effect of As(Ⅲ) on iron-oxidizing bacteria in growth was greater than that on sulfur-oxidizing bacteria. It was found that increasing the bacteria inoculation amount and domesticating bacteria was conducive to the adaptation of moderately thermophilic bacteria to arsenic toxicity. In the actual ore pulp system, increasing the inoculation amount could make moderately thermophilic bacteria finally to adapt to the high arsenic environment and obtain better ore bioleaching effect. The highest mass concentration can reach about 1.54 g/L, and the arsenic leaching rate can be up to 70% in only 10 days.

Key words: bio-oxidation, moderately thermophilic bacteria, valence of arsenic, domestication, bacteria inoculation amount

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