生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (5): 24-.doi: 10.3969/j.issn.2095-1736.2023.05.024

• 研究报告 • 上一篇    下一篇

胶孢炭疽菌侵染秀丽隐杆线虫的特性研究

肖　月1， 易杏盈1， 张东华1， 刘　丽1， 闫晓慧1， 伍建榕1, 2   

  1. 1. 西南林业大学 生物多样性保护学院 云南省高校森林灾害预警控制重点实验室， 昆明 650224；
    2. 西南林业大学 林学院 西南地区生物多样性保育国家林业局重点实验室， 昆明 650224
  • 出版日期:2023-10-18 发布日期:2023-10-17
  • 通讯作者: 张东华，博士，讲师，主要从事资源微生物利用研究，E-mail:1391967127@qq.com
  • 作者简介:肖月，硕士研究生，主要从事森林病理学研究，E-mail:1471564958@qq.com；易杏盈，硕士研究生，主要从事森林病理学研究，E-mail:821995421@qq.com；肖月和易杏盈为共同第一作者
  • 基金资助:
    国家重点研发计划项目(2019YFD100200X)； 国家自然科学基金项目(31860208)； 云南省教育厅项目(11070733)

Study on the infection of Caenorhabditis elegans by Colletotrichum gloeosporioides

XIAO Yue1, YI Xingying1, ZHANG Donghua1, LIU Li1, YAN Xiaohui1, WU Jianrong1, 2   

  1. 1. Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan, College of Biodiversity
    Conservation, Southwest Forestry University, Kunming 650224, China; 2. Key Laboratory of Biodiversity Conservation
    in Southwest China, State Forestry Administration, College of Biodiversity Forestry, Southwest Forestry University,
    Kunming 650224, China
  • Online:2023-10-18 Published:2023-10-17

PDF (PC)

46

摘要： 通过胶孢炭疽菌(Colletotrichum gloeosporioides)和秀丽隐杆线虫（Caenorhabditis elegans）共培养，探讨该植物病原菌对线虫基本生物学特性的影响。结果显示：胶孢炭疽菌对线虫存活率、体长大小、繁殖能力等基本生物学特性产生显著影响，线虫接触胶孢炭疽菌孢子后存活率显著降低，且虫龄大小与其半数致死时间的长短直接相关，L1期为108 h，L4期为144 h；胶孢炭疽菌孢子浓度与线虫存活率呈负相关性；与胶孢炭疽菌共培养的线虫体长大小也明显变短，繁殖能力显著降低；然而，胶孢炭疽菌对线虫运动能力和咽泵速率并无显著影响。结果表明：胶孢炭疽菌对秀丽隐杆线虫可产生一定的伤害作用，具有激活线虫天然免疫系统的可能性，该炭疽菌与线虫互作体系可能成为研究天然免疫进化的有力工具。

关键词: 胶孢炭疽菌, 秀丽隐杆线虫, 存活率, 体长大小, 繁殖能力

Abstract: The effects of the plant pathogen on the basic biological characteristics of Caenorhabditis elegans were studied by co-culture of C. elegans and C. gloeosporioides. The results showed that C. gloeosporioides had great impact on the survival, body length and reproductive of C. elegans. The survival rate of C. elegans decreased obviously after exposing to C. gloeosporioides, and the age of the worm was directly related to the half death time, which was 108 h in L1 stage and 144 h in L4 stage, respectively. The conidia concentration of C. gloeosporioides spores was negatively correlated with the survival of C. elegans. The body length of C. elegans in co-culture with C. gloeosporioides grew shorter evidently, and the reproductive of C. elegans was significantly decreased. However, C. gloeosporioides had no significant influence on C. elegans’ motility capacity and pharynx pump capacity. The results indicated that C. gloeosporioides could harm C. elegans to some extent and might activate the innate immune system of C. elegans. The interaction system between C. gloeosporioides and C. elegans may be a powerful tool for studying the evolution of innate immunity.

Key words: Colletotrichum gloeosporioides, Caenorhabditis elegans, survival, body length, reproductive

中图分类号: