生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (3): 85-.doi: 10.3969/j.issn.2095-1736.2023.03.085

• 研究报告 • 上一篇    下一篇

双壳贝类血浆蛋白质颗粒构成与特性

夏淇峰1,2， 刘优利2,3， 阮紫燕3， 张卫卫1， 林志华2,3， 薛清刚2,3   

  1. 1. 宁波大学 海洋学院， 宁波 315000； 2. 浙江万里学院 宁海海洋生物种业研究院， 宁海 315604；
    3. 浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室， 宁波 315000
  • 出版日期:2023-06-18 发布日期:2023-06-19
  • 通讯作者: 薛清刚，博士，教授，研究方向为贝类宿主防御机制研究，E-mail:qxue@zwu.edu.cn
  • 作者简介:夏淇峰，硕士研究生，研究方向为贝类血浆蛋白构成及功能研究，E-mail:v936041094@163.com
  • 基金资助:
    国家自然科学基金项目（32073010, 31672629）； 宁波市“泛3315”创新团队-海水养殖贝类重要疾病防控技术研究项目； 浙江省自然科学基金项目（LQ22C190006）

Studies on composition and characteristics of protein particles in bivalve hemolymph plasma

XIA Qifeng1,2, LIU Youli2,3, RUAN Ziyan3, ZHANG Weiwei1, LIN Zhihua2,3, XUE Qinggang2,3   

  1. 1. School of Marine Science, Ningbo University, Ningbo 315000, China; 2. Ninghai Institute of Mariculture
    Breeding and Seed Industry, Zhejiang Wanli University, Ninghai 315604, China; 3. Zhejiang Key Laboratory
    of Aquatic Germplasm Resource, Zhejiang Wanli University, Ningbo 315000, China
  • Online:2023-06-18 Published:2023-06-19

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摘要： 选择长牡蛎（Crassostrea gigas）、厚壳贻贝（Mytilus coruscus）、文蛤（Meretrix meretrix）、缢蛏（Sinonovacula constricta）、魁蚶（Scapharca broughtonii）等5种系统分类学有代表性的双壳类物种，利用2种纯化方法结合透射电子显微镜观察，分析血浆蛋白颗粒的存在与否和形态特征。发现除缢蛏外，其他4种双壳贝类的血浆蛋白形成相似的颗粒形态，但大小存在差异。同时，结合SDS-PAGE和氨基酸序列测定鉴定血浆蛋白颗粒的主要蛋白质构成。以长牡蛎血浆蛋白颗粒为代表，观察纯化蛋白颗粒的金属离子解离和结合特征。结果显示：其自然携带的Ca2+可以通过透析排出，在不同质量浓度的金属离子处理时，高质量浓度组的金属结合均高于低浓度组；相同金属离子质量浓度下长牡蛎血浆蛋白颗粒中不同金属离子的含量不同（Cd2+> Pb2+>Ca2+> Zn2+）。双壳贝类物种的多蛋白血浆颗粒有可能通过其金属携带和储运功能参与双壳贝类金属代谢过程。

关键词: 双壳贝类, 血浆蛋白颗粒, 金属结合, 分子筛层析, 金属离子转运

Abstract: The study focused on the function of plasma protein by analyzing the purified plasma protein in five taxonomic representative bivalves(Crassostrea gigas, Mytilus coruscus, Meretrix meretrix, Sinonovacula constricta, and Scapharca broughtonii). The existence and morphological characteristics of plasma protein particles were proved by transmission electron microscope observation. The result showed that the plasma proteins of the four bivalves formed similar particle morphology with different particle sizes, except Sinonovacula constricta. Then, the constituent of the plasma protein particles and total plasma proteins were identified by PAGE. The results of PAGE showed the plasma protein particles of Crassostrea gigas and Mytilus coruscus were mainly consisted of one or two high-density protein bands, while the plasma protein particles of Meretrix meretrix and Scapharca broughtonii consisted of many low-density protein bands. In addition, we analyzed the metal ion dissociation and binding activity of the purified plasma protein particles of the Pacific oyster. The result showed that Ca2+ carried by natural plasma particles of Crassostrea gigas could be separated by dialysis. The particles could absorb more metal ion in the 50 mg/L group than the 5 mg/L group. Under the same metal ion mass concentration, the binding capacity of protein particles between different metal ions showed diversity (Cd2+> Pb2+>Ca2+> Zn2+). The plasma particles might function as the transporting and storing buffer of metal participate in the metal metabolism process of bivalve mollusks.

Key words: bivalves, plasma protein particle, metal binding, molecular sieve chromatography, metal-ions transportation

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