生物学杂志

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代谢中间物对斜生栅藻叶绿素荧光特性以及油脂积累的研究

  

  1. 1. 内蒙古科技大学 生命科学与技术学院, 包头 014010; 2.内蒙古自治区生物质能源化利用重点实验室, 包头 014010; 3. 厦门大学 海洋与地球学院, 厦门 361000;4. 山东省农业科学院 生物技术研究中心, 济南 250000;5. 内蒙古再回首生物工程有限公司, 鄂尔多斯 016100
  • 出版日期:2018-08-18 发布日期:2018-08-18
  • 通讯作者: 季祥,博士,教授,主要从事生物质能、能源微藻研究,E-mail:jixiang@imust.cn
  • 作者简介:成杰,在读博士,主要从事海洋微藻研究,E-mail:15049210053@163.com
  • 基金资助:
    国家自然科学基金(41666007);内蒙古自治区科技计划项目(201601061);内蒙古科技大学大学生创新基金

Effects of metabolic intermediates on chlorophyll fluorescence and total lipid content of Scendesmus obliquus XJ002

  1. 1. School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010; 2. Inner Mongolia Key Laboratory of Biomass-Energy Conversion, Baotou 014010;3. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361000;4. Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250000;5. Inner Mongoiia Rejuve Biotech Limited Company, Ordos 016100, China
  • Online:2018-08-18 Published:2018-08-18

摘要: 为了研究代谢中间物(柠檬酸与苹果酸)的添加对斜生栅藻(Scenedesmus obliquus XJ002)光合效率以及胞内油脂积累的影响,研究了代谢中间物的添加对XJ002藻株生物量、光合色素、叶绿素荧光特性以及胞内总脂含量的影响。结果表明:添加高浓度代谢中间物导致藻细胞生物量与光合色素含量显著下降,损坏藻细胞光系统II反应中心供体侧的放氧复合体(OEC)结构,抑制光系统II反应中心受体侧电子传递活性。当柠檬酸与苹果酸处理浓度为0.15 g/L时,XJ002藻株胞内总脂含量是对照组的1.85 倍和1.34 倍。高浓度代谢中间物的添加,显著提高了XJ002藻株胞内总脂含量,与苹果酸相比,柠檬酸的添加效果更佳。

关键词: 柠檬酸, 苹果酸, 斜生栅藻, 叶绿素荧光, 总脂

Abstract: In order to study the effects of exogenous metabolic intermediates on photosynthetic efficiency and total lipid content in Scenedesmus obliquus XJ002, we added metabolic intermediates (citric acid and malic acid)to study biomass, photosynthetic pigments, chlorophyll fluorescence characteristics and lipid content of XJ002. The results showed that the addition of high concentrations of metabolic intermediates resulted in a significant decrease in biomass, chlorophyll a, chlorophyll b and carotenoid content. The high concentration treatments could damage the oxygen evolving complex at the donor side of reaction center of photosystem II, and inhibited electron transfer activity of receptor side of reaction center of photosystem II. The addition of intermediate metabolites resulted in a significant increase in the total lipid content of XJ002. When the concentration of citric acid and malic acid was 0.15 g/L, the total lipid content was 1.85 and 1.34 folds higher than the control group. The addition of high concentration of metabolic intermediates results in a significant increase in total lipid content in the cell of XJ002. Compared with malic acid, citric acid has the better effect.

Key words: citric acid, malic acid, Scendesmus obliquus, chlorophyll fluorescence, total lipid