生物学杂志

• 研究报告 • 上一篇    下一篇

温度和光照强度对浒苔生长和光合生理特性的影响

  

  1. 1. 宁波大学 海洋学院, 宁波 315211; 2. 浙江省海洋生物工程重点实验室, 宁波 315211
  • 出版日期:2018-08-18 发布日期:2018-08-18
  • 通讯作者: 李亚鹤,讲师,博士,研究方向为藻类生理生态,E-mail: liyahe@nbu.edu.cn
  • 作者简介:孟晓智,研究方向为藻类生理生态,E-mail:751992603@qq.com
  • 基金资助:
    国家自然基金项目(41606129);浙江省自然科学基金(LQ15D060002)

Combined effects of temperature and light intensity on the growth and physiological performances of Ulva prolifera

  1. 1. School of Marine Sciences, Ningbo University, Ningbo 315211;2. Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China
  • Online:2018-08-18 Published:2018-08-18

摘要: 实验以浒苔(Ulva prolifera)为材料,设置3个温度(20℃、25℃及30℃)和两个光照强度(低光:70 μmol/m2·s;高光:250 μmol/m2·s),通过测定不同培养条件下浒苔的生长、叶绿素含量、光合荧光参数、呼吸速率及光合放氧速率,初步探讨温度和光照强度对浒苔生长和光合特性的复合效应,为研究浒苔绿潮爆发及规模化养殖提供一定的参考。结果显示:1)光照强度的升高显著促进浒苔的生长;温度从20℃升高至25℃对浒苔生长的影响不显著,但升高至30℃具有显著的抑制作用。2)30℃、250 μmol/m2·s条件下,浒苔的有效光化学效率(Fv′/Fm′)及最大相对传递速率(rETRmax)显著降低。3)高光条件下,25℃时浒苔具有较高的暗呼吸(Rd)及净光合放氧速率(Pn);20℃时浒苔的暗呼吸和总光合放氧速率的比值(Rd/ Pg)最低。整体来说,光照强度的适度增加提高了浒苔对升温的适应。

关键词: 浒苔, 光照强度, 光合放氧, 叶绿素荧光参数, 温度

Abstract: In order to study the combined effects of the light and temperature on the growth and physiological performance, the chosen Ulva prolifera grew under three temperature levels (20, 25 and 30℃) and two light conditions (70, 250 μmol/m2·s), then the parameters like the growth rate, Chl a content, chlorophyll fluorescence parameters, dark respiration and photosynthetic oxygen evolution rate were determined. The results showed that the growth of U. prolifera was enhanced by higher light; there were no significant difference in the growth rates between 20 and 25℃ treatments, but the growth was significant inhibited at 30℃. The effective quantum yield (Fv′/Fm′) and the maximal relative electron transfer rate (rETRmax) were lowest under the 30℃ and 250 μmol/m2·s treatment. U. prolifera showed higher dark respiration rate (Rd) and net photosynthetic oxygen evolution rate (Pn) at 25℃ under high light level with lowest value in Rd/Pn. In one word, the effects of higher temperature on U. prolifera were mediated by light levels.

Key words: Ulva prolifera, light intensity, photosynthetic oxygen evolution, chlorophyll fluorescence parameters, temperature