生物学杂志

生物学杂志

• 研究报告 • 上一篇    下一篇

膀胱癌临床血清样本N-连接糖链的表达分析

  

  1. 1. 江南大学 生物工程学院 糖化学与生物技术教育部重点实验室， 无锡 214122；2. 江南大学 附属医学院泌尿科， 无锡 214122
  • 出版日期:2017-10-18 发布日期:2017-10-18
  • 通讯作者: 关锋，博士，教授，研究方向为糖生物学，E-mail: fengguan@jiangnan.edu.cn
  • 作者简介:王雯雯，硕士研究生，专业方向为轻工技术与工程，E-mail: 6140206015@vip.jiangnan.edu.cn
  • 基金资助:
    国家自然科学基金(81672537); 国家自然科学基金(81402115)

Analysis of N-linked glycans in bladder cancer

  1. 1. Key Laboratory of Carbohydrate Chemistry ＆ Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122; 2. Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi 214122, China
  • Online:2017-10-18 Published:2017-10-18

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摘要： 膀胱癌是发生在膀胱黏膜上的恶性肿瘤，是最常见的泌尿系统肿瘤之一。国内外研究表明糖链表达的异常改变与肿瘤的发生、发展和转移紧密关联。分析膀胱癌临床样本糖链的表达变化可以为膀胱癌研究提供有效的分析手段，有助于发现潜在的临床早期诊断标志物。首先利用乙酰肼修饰糖链唾液酸，用PNGase F酶将糖蛋白质N-连接糖链释放出来，通过基质辅助激光解析电离飞行时间质谱(MALDI-TOF/TOF-MS)技术对20例膀胱癌血清，11例正常人血清中糖蛋白N-连接糖链进行相对定量分析。共鉴定出42种N-连接糖链结构，其中15种N-连接糖链在两组样本中含量差异明显。此外，通过分析不同类型的糖链在两组临床样本中的分布差异，发现唾液酸化、复合型的N-连接糖链在膀胱癌血清中呈现显著升高的趋势，而高甘露糖型、平分型N-连接糖链表达呈下降趋势，说明这些糖链结构的表达变化与膀胱癌的发生有关，从而有助于进一步阐明膀胱癌发生过程中糖链相关的分子机理，并可为膀胱癌的早期诊断、恶性演进和转移预测提供数据支持。

关键词: 膀胱癌, 血清, N-连接糖链, 生物标志物, MALDI-TOF/TOF-MS

Abstract: Bladder cancer is one of the most common cancers and which occurs in the bladder mucosa.The aberrant changes of glycans have been shown to be associated with tumorigenesis, tumor progression and metastasis. The study focused on structures and functions of tumor-specific glycans was useful for the discovery of new candidate biomarkers of bladder cancer. In this study, N-glycans in glycoproteins in 20 bladder cancer and 11 normal samples were released by PNGase F, and sialic acids were modified by acetohydrazide. A total of 42 N-glycan structures were identified and 15 N-glycans were thought to be candidate biomarkers. It indicated that complex type and sialylation type N-glycans were increased significantly, while the high-mannose type and bisecting N-glycans were decreased significantly in bladder cancer sera. These findings showed the expression of glycan structure is associated with the occurrence of bladder cancer, which is important to reveal the glycan mechanism in the development of bladder cancer. The differential glycans could be useful for the early diagnosis. 

Key words: bladder cancer, serum, N-linked glycan, biomarkers, MALDI-TOF/TOF-MS